Fibronectin, a fibroblast surface protein, was purified from human and chicken plasma and extracts of cultured chicken fibroblasts with affinity chromatography on gelatin coupled to Sepharose particles. A fibronectin-like protein was also isolated from the plasma of Torpedo fish. The collagen binding properties of fibronectin were studied with several genetically distinct collagens. Heat denatu...

An epitope-specific polyclonal antiserum was produced in rabbits immunized against a synthetic 15 amino acid peptide (QRVTGLDFIPGLHPV) derived from the coding sequence reported for the porcine leptin gene (GenBank Accession No. U59894). This peptide contains a core sequence comprised of eight amino acids (GLDFIPGL) that is totally conserved in all leptin proteins studied to date. Purified recom...

Native Dang Surat chicken play an important role in poultry breeding of southern Thailand but their production performance falls short commercial counterparts. Nevertheless, native birds have superior disease resistance while meat texture and taste eggs are popular among Thais. Husbandry breeds confined to smallholders who can only fulfil local niche outlets. To satisfy the wide-spread demand t...

The effect of reversible inactivation on the physical and chemical properties of dogfish M4 and chicken heart H* lactate dehydrogenases has been studied. Reduced diphosphopyridine nucleotide was found to have a significant effect on the rate of recovery of the enzymatic activity, but not on the over-all recovery. Optical rotatory dispersion, circular dichroism, fluorescence, and immunological s...

Sarcomeric mitochondrial creatine kinase (Mib-CK) of chicken was expressed in Escherichia coli as a soluble enzyme by using an inducible phage-T7 promoter. Up to one third of the protein in E. coli extracts consisted of soluble recombinant Mib-CK in an enzymically active form. Approx. 20 mg of nearly-homogenous Mib-CK was isolated in a two-step isolation procedure starting with 1 litre of isopr...

Domestic chicken (Gallus domesticus) is one type of poultry that has experienced domestication and spread throughout the archipelago. Chicken consumed by humans should be in a healthy condition free from various types parasites. Information about infected with parasites needed to maintain animal health prevent occurrence zoonotic diseases. Research been carried out on Karang Bongkot Village, La...

The genetic diversity of native chicken populations from Myanmar, Thailand, and Laos was examined by using 102 insertion and/or deletion (indels) markers. Most of the indels loci were polymorphic (71% to 96%), and the genetic variability was similar in all populations. The average observed heterozygosities (H O ) and expected heterozygosities (H E ) ranged from 0.205 to 0.263 and 0.239 to 0.381...

Efforts that can be made to improve the performance of native chickens are by crossing them with Bangkok chickens. This study aims determine growth body weight from cross-breeding between Kampung chicken and chicken. As many as 30 male chicks a cross were used in this study. The crosses rooster chicks. DOC female was 35.22 ± 0.96 grams. There significant increase starting at age 5 weeks 8 age, ...