A single-step concentration and elution method is developed for detection of DNA in buffer, saliva, and blood. A nanotip capturing DNA using an electric field and capillary action is directly dissolved in buffer for qPCR analysis. The concentration yield and the relative parameters are compared with those of a commercial kit.

Different approaches to obtain sharp silicon tips with a variety of aspect ratios, for potential use in advanced microelectronics applications, were studied. Tips suited for atomic force microscopy and field emission arrays were formed by wet chemical etching of (100) and (111) single crystal silicon in KOH, TMAH and HNA etchant. Apex sharpening with thermal oxidising step resulted in tips with...

We present HST/NICMOS imaging of the H2 2.12 μm emission in 5 fields in the Helix Nebula ranging in radial distance from 250–450 from the central star. The images reveal arcuate structures with their apexes pointing towards the central star. These molecular hydrogen knots are most highly structured in the fields closest to the central star and become increasingly less structured with increasing...

PURPOSE To correlate parameters derived from corneal deformation resulting from non-contact tonometry integrated with an ultra-high-speed (UHS) Scheimpflug camera (Oculus Corvis ST, Scheimpflug Technology; Wetzlar, Germany) with age in normal eyes from young patients. METHODS Observational, retrospective study involving one eye randomly selected from study participants, totaling 89 healthy ey...

Limpets are commonly found on wave-swept rocky shores, where they may be subjected to water velocities in excess of 20 m s(-1). These extreme flows can impose large forces (lift and drag), challenging the animal's ability to adhere to the substratum. It is commonly thought that the conical shape of limpet shells has evolved in part to reduce these hydrodynamic forces while providing a large ape...

Microscopic ion fluxes in plasma-aided nanofabrication of ordered carbon nanotip structures I. Levchenko and K. Ostrikov School of Physics, The University of Sydney, Sydney, New South Wales 2006, Australia M. Keidar Department of Aerospace Engineering, University of Michigan, Ann Arbor, Michigan 48109-2140 S. Xu Plasma Sources and Applications Center, National Institute of Education (NIE), Nany...

A case is presented of a 78-year-old woman who sustained a re-fracture of the distal radius after initial fixation with a volar locking plate. The fracture was located in the mid-portion of the volar plate with palmar apex bending of the plate and signs of median nerve compression. A successful closed reduction was performed without hardware failure. The fracture united uneventfully.

OBJECTIVE The aim of ideal canal preparation is to prevent iatrogenic aberrations such as transportation. The aim of this study was to evaluate the root canal transportation by Reciproc file with and without glide path. MATERIALS AND METHODS Thirty acrylic-resin blocks with a curvature of 60° and size#10 (2% taper) were assigned into two groups (n= 15). In group 1, the glide path was performe...

3 Rady Children’s Hospital, UCSD, San Diego, CA Many cast wedging techniques have been discussed in the literature; however, to our knowledge, these techniques have not been experimentally validated. This paper illustrates a technique and measurement methodology for reproducible wedging and validates the geometric approach to predict the correct wedge size. Fracture deformities of saw bone mode...