the aim of this study was to prepare liposomes of acyclovir (acy) by thin layer evaporation (tle) and reverse phase evaporation (rev) methods. twenty-seven batches of liposomes from each method were prepared using technique of three variables at three levels (33) factorial design. drug/lipid (molar ratio), hydration volume and hydration time were considered three independent variables in tle me...

Lipid vesicles have received significant attention in areas ranging from pharmaceutical and biomedical engineering to novel materials and nanotechnology. Microfluidic-based synthesis of liposomes offers a number of advantages over the more traditional synthesis methods such as extrusion and sonication. One such microfluidic approach is microfluidic hydrodynamic focusing (MHF), which has been us...

The high incidence of bacterial infection and the growing resistance of bacteria to conventional antibiotics have resulted in the strong need for the development of new generation of antibiotics. Nano-sized particles have been considered as novel antibacterial agents with high surface area and high reactivity. The overall antibacterial properties of antimicrobial nanostructures can be significa...

Exosomes are 40-100 nm nano-sized vesicles that are released from many cell types into the extracellular space. Such vesicles are widely distributed in various body fluids. Recently, mRNAs and microRNAs (miRNAs) have been identified in exosomes, which can be taken up by neighboring or distant cells and subsequently modulate recipient cells. This suggests an active sorting mechanism of exosomal ...

Fabric softeners are widely used to make clothes soft and impart nice smell. Household conditioners water-based formulations consisting of quaternary ammonium surfactants assembled in vesicles. On the other hand, for textiles finishing typically silicone oil emulsions. Here, these technologies combined a novel industrial formulation which contains both surfactant vesicles oil. In particular, we...

methods mycobacterium tuberculosis standard strain crbip7.11 was cultured at 37°c in loewenstein johnson (lj) media for 3-4 weeks. to confirm the species, standard microbiological and biochemical tests were performed. after preparation of membrane vesicles, the amount of protein in membrane vesicles was measured by sds-page and nanodrop. to analyze the integrity and morphology of extracellular ...

Melittin, the principal constituent in bee venom, is an attractive candidate for cancer therapy. However, its clinical applications are limited by hemolysis, nonspecific cytotoxicity, and rapid metabolism. Herein, a novel genetically engineered vesicular antibody-melittin (VAM) drug delivery platform was proposed validated targeted combination VAM generated from cellular plasma membrane bio-syn...

Extracellular vesicles are membrane-derived nanoparticles that represent a novel mechanism of cell-to-cell communication. It is well reported EVs play central role in the tumor microenvironment by mediating intercellular signaling among cancer cells. This has resulted development therapeutic strategies targeting various EV pathways cancer. However, because their small size and endogenous origin...

BACKGROUND The purpose of this study was to formulate and evaluate nano lipid vesicles of methotrexate (MTX) for its anti-rheumatoid activity. METHODS In this study the principle of both active as well as passive targeting using MTX-loaded stealth liposomes as per the magic gun approach was followed. Stealth liposomes of MTX were prepared by thin-film hydration method using a PEGylated phosph...