In developing countries where tuberculosis is still a health challenge, the prevalence of nontuberculous mycobacterial diseases is expected to rise as medical conditions that compromise the immune system become more widespread. In the current study, we aimed to determine the presence and diversity of nontuberculous mycobacteria (NTM) causing infections in Iranian patients. Sixty-seven clinical ...

Direct examination † Culture Molecular identification Ring of epithelial cells Giant cells Caseous necrosis Altered leukocytes Reference M. tuberculosis ± + IS6110 + + + – (6) M. avium 183 NR NR NR + NR + + NR NR (7) M. avium subsp. hominissuis 34 NR NR NR + IS1245 NR NR NR NR (5) M. haemophilum 39 NR NR + + 16S–23S rDNA + + + – (8) M. lentiflavum 3 SE Cured + + 16S rRNA + + NR + (9) M. bohemic...

Accurate identification of slowly growing nontuberculous mycobacteria (SG-NTM) of clinical significance remains problematic. This study evaluated a novel method of SG-NTM identification by amplification of the mycobacterial 16S-23S rRNA internal transcribed spacer (ITS) region followed by resolution of amplified fragments by sequencer-based capillary gel electrophoresis (SCGE). Fourteen America...

BACKGROUND Non-Tuberculous Mycobacteria (NTM) are emerging around the world due to a higher prevalence of immunosuppressive illness and therapy. Saudi Arabia is not an exception as there have been novel mycobacterial species also identified. In addition, several published case reports from different parts of the country suggest a growing pathogenic potential of NTM. As the first nationwide stud...

Drinking water distribution systems were analyzed for viable counts of mycobacteria by sampling water from waterworks and in different parts of the systems. In addition, loose deposits collected during mechanical cleaning of the main pipelines were similarly analyzed. The study covered 16 systems at eight localities in Finland. In an experimental study, mycobacterial colonization of biofilms on...

BACKGROUND Several nontuberculous mycobacteria (NTM) were previously isolated from diverse environments such as water, soil, sewage, food and animals. Some of these NTM are now known to be opportunistic pathogens of humans. OBJECTIVE The main purpose of the study was to identify NTM isolates stored at the National Microbiology Reference Laboratory (NMRL) and were previously isolated from huma...

The identification of Mycobacteria in the species level has great medical importance. Biochemical tests are laborious and time-consuming, so new techniques could be used to identify the species. This research aimed to the comparison of biochemical and sequencing 16S rDNA gene methods to identify nontuberculous Mycobacteria in patients suspected to tuberculosis in Golestan province which is the ...

Background: Diabetes is an important metabolic disease with myriad manifestations and complications, affecting many people. Cutaneous infections impact numerous diabetic patients. Among different bacterial infections in diabetic patients, the infections caused by mycobacteria other than Mycobacterium tuberculosis (MOTT) eventuate in complications owing to the paucity of accurate detection...

background and objectives: mycobacterium tuberculosis complex is consisted of homogenous organisms. they are slowly growing mycobacteria and their isolation and identification are difficult and time consuming. differentiation of mycobacterium bovis, causative mammalian tuberculosis, from other members of mycobacterium tuberculosis complex is very important in epidemiology and control of disease...