Ca2+, Mg2+-ionophores X537A and A23,187 (10(-7)-10(-6) M) induced the release of adenine nucleotides adenosine diphosphate (ADP, adenosine triphosphate (ATP), serotonin, beta-glucuronidase, Ca2+, and Mg2+ from washed human platelets. Enzymes present in the cytoplasm or mitochondria, and Zn2+ were not released. The rate of ATP and Ca2+ release measured by firefly lantern extract and murexide dye...

A spectre-photometric method is described for the determination of magnesium* calcium, strontium and barium coexisting in binary mixtures. The method employs totramethyimurexide as an auxiliary ligand, and has been developed for pure two-component systems, such as are involved in investigations of cation-exchange equilibria on anionic polymers. The analysis is performed with calibration curves ...

The effects of external anions on gating of Na channels of frog skeletal muscle were studied under voltage clamp. Anions reversibly shift the voltage dependence of peak sodium permeability and of steady state sodium inactivation towards more negative potentials in the sequence: methanesulfonate less than or equal to Cl- less than or equal to acetate less than Br- less than or equal to NO-3 less...

AB S TRACT The effects of external anions on gating of Na channels of frog skeletal muscle were studied under voltage clamp. Anions reversibly shift the voltage dependence of peak sodium permeability and of steady state sodium inactivation towards more negative potentials in the sequence : methanesulfonate < Cl5 acetate < Br5N03 < S04 < benzenesulfonate < SCN< C104-; approximately the lyotropic...

We studied the effects of caffeine on calcium transport by subcellular organelles isolated from rabbit myocardium. Caffeine increased myofibrillar basic and calcium-activated ATPase activity at 20 mM but not at lower concentrations. Mitochondrial and sarcoplasmic reticulum (SR) calcium accumulation was measured both by dual wavelength spectrophotometry with the calcium-sensitive dye, murexide, ...

Visceral urate deposition (visceral gout) is a common finding during post-mortem examination of poultry. Rare cases of visceral gout may occur in wild birds. A rare case of visceral urate deposition in a little bittern (Ixobrychus minutus) is reported here. In May 2013, carcass of a little bittern was submitted for necropsy to the Clinic of Poultry Diseases (Faculty of Veterinary Medicine, Razi...

The standard method for the determination of serum calcium based upon the precipitation of calcium oxalate and titration with permanganate does not lend itself to a micromethod because of the difficulty in handling very small precipitates. This difficulty is not encountered in the direct titration of calcium with the chelating agent, disodium dihydrogen ethylenediaminetetraacetate (EDTA). Schwa...

Alloxan is a polyoxy-pyrimidine of unusual biological interest, being the agent of choice for producing experimental diabetes (Shaw-Dunn, Sheehan, and McLetchie, 1943). Despite the fact that it has been used extensively for many years, very little is known of its ultimate fate in mammals or other forms of life. It is exceedingly unstable at physiological pH values and is spontaneously converted...