Drug resistance in Plasmodium falciparum is a serious public health threat in the countries where this organism is endemic. Since resistance has been associated with specific single-nucleotide polymorphisms (SNPs) in parasite genes, molecular markers are becoming useful surrogates for monitoring the emergence and dispersion of drug resistance. In this study, a multiplex PCR (mPCR) and oligonucl...

Regarding food safety, rapid detection of microbial species is crucial to develop effective preventive and/or adjustment measures. Classical methods for determining the presence of certain species are time-consuming and labor-intensive, hence, molecular methods, which offer speed, sensitivity and specificity, have been developed to address this problem. Multiplex PCR (MPCR) is widely applied in...

A total of 112 fungal isolates were recovered from cereal grains collected from 10 districts of Tamilnadu and Andhra Pradesh states. The collected samples were subjected to standard blotter method and different ochratoxigenic Aspergillus and Penicillium species were isolated and maintained. A multiplex PCR assay was standardized for simultaneous detection of toxigenic Aspergillus and ochratoxin...

We describe the first isolation of an enteroaggregative Escherichia coli (EAEC) O104:H4 strain associated with an acute diarrhea case in Argentina. Two multiplex PCRs (mPCR) were performed as screening of genes mPCR1 (eae, lt, and st) and mPCR2 (IpaH, aggR, stx1 and stx2). A mPCR to detect the rfbO104, fliCH4 and terD genes, and PCR assays for the detection of pCVD432 plasmid, aaiC and lpfO113 ...

In the study nasal and throat swabs were collected from 78 apparently healthy and 69 cases of kennel cough from dogs of different age, sex, breed and places and analysed for presence of Bordetella bronchiseptica through bacterial isolation and multiplex PCR (mPCR). The pathogen could be isolated from an apparently healthy Labrador bitch while with mPCR, 7 (4.76%, 4 from healthy dogs and 3 from ...

Pasteurella multocida is a Gram-negative bacterial pathogen that is the causative agent of a wide range of diseases in many animal species, including humans. A widely used method for differentiation of P. multocida strains involves the Heddleston serotyping scheme. This scheme was developed in the early 1970s and classifies P. multocida strains into 16 somatic or lipopolysaccharide (LPS) serova...

Most mycobacterial infections are still caused by Mycobacterium tuberculosis complex (MTC) strains; however, infections by non-tuberculous mycobacteria (NTM) are increasing, particularly among immunocompromised patients. Conventional species-specific identification and proper patient management are delayed due to the slow-growing nature of mycobacteria. We have developed a multiplex PCR (mPCR) ...

INTRODUCTION: This study aimed to determine the prevalence, and virulence factors of Listeria monocytogenes isolated from various samples by multiplex polymerase chain reaction (MPCR). METHODS: A total of 617 isolates were obtained and MPCR was employed for detection of the inlA, inlC, and inlJ genes. RESULTS: L. monocytogenes was detected in 46 (7.45%) of the 617 specimens. inlA, inlC, a...

Latar Belakang: Lepra adalah penyakit purba yang disebabkan oleh Mycobacterium leprae (M.leprae). Indonesia masih menjadi penyumbang kasus lepra tertinggi kedua di dunia. Kasus ada kerap kali terdiagnosis ketika pasien sudah memiliki gejala klinis parah. Tujuan: Penelitian ini bertujuan untuk mengetahui Potensi Nasal Swab mPCR diperkuat dengan MNP dan CL target gen RLEP, 16SrRNA sodA sebagai me...