We discuss the short-time response of a multicellular spheroid to an external pressure jump. Our experiments show that 5 min after the pressure jump, the cell density increases in the centre of the spheroid but does not change appreciably close to the surface of the spheroid. This result can be explained if the cells are polarized which we show to be the case. Motivated by the experimental resu...

A technique has been developed which takes advantage of the spherical symmetry of EMT6/Ro multicellular tumor sphe roids to isolate subpopulations of cells originating from various locations in the spheroid structure. The method involves gently exposing spheroids to a dilute trypsin solution at 18 to 20°in specially designed dishes on a rotary shaker. Under these conditions, cells are released...

Hypoxia is an important characteristic of malignant solid tumors and is considered as a possible causative factor for serious resistance to chemo- and radiotherapy. The exploration of novel fluorescent probes capable of detecting hypoxia in solid tumors will aid tumor diagnosis and treatment. In this study, we reported the design and synthesis of a series of "off-on" phosphorescence probes for ...

In this paper, a microfluidic system for long-term multicellular tumor spheroids culture and analysis is presented. Human carcinoma cells were cultured in an array of microwells for four weeks and a slowdown of the proliferation with maintained viability was observed. The construction of the device enabled observation of decreasing spheroid diameter as an indicator of cell death. Long-term obse...

The multicellular spheroid model partly mimics tumor microenvironments in vivo and has been reported in plenty of studies regarding radiosensitivity. However, clear isolation of quiescent and proliferating cells in live conditions has been quite difficult owing to technical limitations; therefore, comprehensive characterization could not be done thus far. In this study, we succeeded in separate...