We studied the serum levels of interleukin-10 (IL-10), in patients with adult T-cell leukemia (ATL) caused by human T-cell leukemia virus type I (HTLV-I) infection. Elevated IL-10 levels were observed in 33 of 45 patients with ATL. Fresh leukemic cells from ATL patients as well as HTLV-I-infected T-cell lines MT-2, SLB-1, and C10/MJ expressed IL-10 mRNA by reverse transcription-polymerase chain...

The objective of this study was to evaluate boron tolerance wheat genotypes using mature embryo culture. analysis involved 79 recombinant inbred lines the International Triticeae Mapping Initiative (ITMI) population and three Serbian varieties with known (B) (Pobeda - sensitive S, Balerina-medium tolerant MT, Nevesinjka T). evaluation performed on a modified MS medium which 15 mM boric acid add...

Most of the plant regeneration processes in citrus, through tissue culture, involve indirect somatic embryogenesis. The optimization of these processes is important for the development of in vitro plant improvement and micropropagation studies. Studies to evaluate the effect of different carbohydrates in somatic embryogenesis were conducted using calli from ‘Ponkan’ mandarin (Citrus reticulata,...

background and objectives: tuberculosis (tb) is a major problem in the world. treatment and control of tb needs detection of the mycobacterium tuberculosis (mt) in the proper samples. while smear doesn’t have enough sensitivity, culture and pcr are expensive, time consuming and unavailable in many centers. recent development of a rapid tb antigen detection test (prtbk) at pasteur institute of i...

During our continuing search for new biologically active metabolites from myxobacteria1*, a new screening system using an MT-4cell culture assay with HIV-12'3) led to the discovery of the novel secondary metabolite, phenoxan (Fig. 1). It was isolated from an acetone-extract of the cell mass of Polyangium spec, strain PI VO19 and purified as described elsewhere4*. The chemical and spectroscopic ...

Leukemia inhibitory factor (LIF), similar to interleukin-6 (IL-6), is a glycoprotein growth factor and differentiation regulator that has pleiotropic activity in several cellular systems. Recent reports of constitutive IL-6 production from spontaneously proliferating cells from human T-cell leukemia virus (HTLV)-infected individuals led us to examine the expression of IL-6 and LIF during HTLV i...

We examined the morphological changes in mitochondria, mitochondrial nucleoids (mt-nucleoids), and actin cytoskeleton yeast Saccharomyces cerevisiae during transition from anaerobic to aerobic culture by immunofluorescence microscopy, DAPI staining, rhodamine-phalloidin staining. The cells grown anaerobically stationary phase contained a small number of 7.3 per cell on average. When were transf...