The use of liquid chromatography (LC) coupled with tandem mass spectrometry (MS/MS) or single-stage mass spectrometry (MS) is making significant inroads in the analyst's compendium of instrumentation available for the analysis of drugs in biological fluids, tissues, and other specimens of interest. LC/MS(/MS) has the unique capability of analyzing substances frequently not analyzable by any oth...

SWATH-MS is an acquisition and analysis technique of targeted proteomics that enables measuring several thousand proteins with high reproducibility and accuracy across many samples. OpenSWATH is popular open-source software for peptide identification and quantification from SWATH-MS data. For downstream statistical and quantitative analysis there exist different tools such as MSstats, mapDIA an...

Amidopyrene-conjugated compounds can be detected by label-assisted laser desorption/ionization mass spectrometry (LA-LDI MS) without matrixes. When actin, a cytoskeletal protein, was labeled with an excess amount of amidopyrene N-hydroxysuccinate (apy-OSu), eight apy-labeled actin peptides were predominantly detected by LA-LDI MS. Then actin was labeled with an amidopyrene NHS ester of the anti...

The development of modern mass spectrometry (MS) equipment with high resolution and mass accuracy has led to its use in analyzing glycans for both profiling and structural studies. Unlike the HPLC method, a substantially larger amount (~10‐20 times) of glycan is normally required for a single MS spectrum. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry i...

A novel liquid chromatography–tandem mass spectrometry (LC–MS/MS) method with a dual electrospray ionization (ESI) and atmospheric pressure chemical (APCI) source was developed for analyzing 102 pesticides five mycotoxins that are regulated by the state of Colorado in hemp. The limit quantitation (LOQ) met action requirements these analytes hemp matrix. For this study, 88 out all were analyzed ...

In this paper, we describe the processing and verification work undertaken to migrate WordStar for MSDOS to HTML4 formatted files. General Terms Preservation strategies and workflows, specialist content types, digital preservation marketplace, theory of digital preservation, case studies and best practice.

Piracetam was subjected to the hydrolytic, oxidative, thermal and photolytic stress, according to ICH guideline Q1A (R2). The drug depicted degradation only in basic conditions, while it was stable to other stress conditions (acidic, neutral hydrolysis, thermal and photolytic conditions). A total of one degradation product was formed, which were separated on a BEH column employing an isocratic ...

Atypical or idiosyncratic language is a characteristic of autism spectrum disorder (ASD). In this paper, we discuss previous work identifying language errors associated with atypical language in ASD and describe a procedure for reproducing those results. We describe our data set, which consists of transcribed data from a widely used clinical diagnostic instrument (the ADOS) for children with au...