Hexabromocyclododecane (HBCD, C12H18Br6) is the most widely used of the cycloaliphatic BFRs. In 1999, the global demand for HBCD (~16 kilotons) was more than double that of the penta-bromodiphenyl ether (~8.5 kilotons) mixture [1]. HBCD is used as thermal insulation in the building industry, and is the principal flame retardant in extruded (XEPS) and expanded (EPS) polystyrene foams [2]. Second...

Metabolic flux analysis (MFA) is a widely used method for quantifying intracellular metabolic fluxes. It works by feeding cells with isotopic labeled nutrients, measuring metabolite isotopic labeling, and computationally interpreting the measured labeling data to estimate flux. Tandem mass-spectrometry (MS/MS) has been shown to be useful for MFA, providing positional isotopic labeling data. Spe...

GC/MS/MS techniques to quantitate mixtures of acyclic stereoisomers are explored for a set of secondary n-alkyl phenyl ethers vicinally substituted with deuterium. Examination of a set of test compounds using a commercial quadrupole ion trap instrument (GC/QIT) reveals large differences between threo and erythro diastereomers when trapped molecular ions expel neutral alkene as a result of colli...

In order to overcome many limitations of immunoassays, high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) has the potential to find its place in the clinical laboratory medicine for quantification of steroid hormones. A prerequisite for the application of a new analytical procedure in clinical diagnostics is standardization to minimize analytical intra- and interlabora...

LC/MS/MS is a very selective and sensitive analytical technique for detection and quantification of analytes in complex matrices. As a result, the use of LC/MS/MS in forensic toxicological applications has been significantly increasing over the past decade. The use of oral fluid as an alternative biological matrix for drug testing has also increased. However, the sample volume is usually much s...

Metabolomics aims at the detection and quantitation of metabolites within a biological system. As the most direct representation of phenotypic changes, metabolomics is an important component in system biology research. Recent development on highresolution, high-accuracy mass spectrometers enables the simultaneous study of hundreds or even thousands of metabolites in one experiment. Liquid chrom...

The isomers of the Man(7)GlcNAc(2) glycan obtained from bovine ribonuclease B have been characterized by ion mobility spectrometry-tandem mass spectrometry (IMS-MS/MS). In these experiments, [Man7 + 2Na](2+) precursors having different mobilities are selected by ion mobility spectrometry and analyzed by MS/MS techniques in an ion trap. The fragmentation spectra obtained for various precursor io...