(SCNT) embryos of buffaloes. 2. To study gene expression profile of important developmental genes at different stages of SCNT cloned embryo. 3. To study epigenetic reprogramming during early developments of SCNT embryos Materials and Methods: Expression analysis of developmental genes was done in different (ovarian granulose and cumulus and skin fibroblasts) donor cells; in vitro maturing oocyt...

Runx3, a member of Runt-related transcription factor (Runx) proteins with tumor suppressor effect, is a tissue–restricted and cancer related transcription factor that regulate cell proliferation and growth, as well as differentiation. In the present study, exogenous Run3 was transiently expressed in AGS (human gastric adenocarcinoma), with undetectable Runx3 protein and in A549 (human lung carc...

background: the aim of this study is to create an ex vivo model to examine the expression of two heat shock protein 70 (hsp70) family members, heat shock protein 72 (hsp72) and heat shock constitute protein 70 (hsc70), at the mrna and protein levels in differentiating corneal cells from air exposed limbal stem cells. materials and methods: limbal biopsies were cultured as explants on a cellular...

Bakground: Multiple sclerosis (MS) is a CD4+ T cell-mediated autoimmune disease affecting the central nervous system (CNS). It was previously believed that Th1 cells were pathogenic T cells in experimental autoimmune encephalomyelitis (EAE). However, the functional role of Th1 cells in EAE has been reconsidered upon the discovery of IL-17- producing T cells which are consider as dominant effect...

background: this study detected osteopontin (opn) and matrix metalloproteinase-7 (mmp-7) expressions to explore the roles of opn and mmp-7 in the occurrence, progression, and prognosis of nonsmall cell lung cancer (nsclc). materials and methods: a retrospective study was conducted on nsclc tissues ( n = 152; case group) and adjacent nonneoplastic lung parenchyma (adjacent to tumor >5 cm; n = 15...

Background Although aberrant protamine (PRM) ratios have been observed in infertile men, the mechanisms that implicit the uncoupling of PRM1 and PRM2 expression remain unclear. To uncover these mechanisms, in this observational study we have compared the PRM1/PRM2 mRNA ratio and mRNA contents of two regulatory factors of these genes. MaterialsAndMethods In this experimental study, sampling was ...

Objective(s): Several pathological conditions are associated with hyper-production of testosterone; however, its impacts are not well understood. Hence, we evaluated the effects of supraphysiological levels of testosterone on gonadotropin-releasing hormone (GnRH) system in the hypothalamus of male rats. Also, we assessed the expression of two excitatory (kisspeptin and...

Elevated expression of the drug efflux transporter ABCG2 seems to correlate with multidrug resistance of cancer cells. Specific COX-2 inhibitor celecoxib has been shown to enhance the sensitivity of cancer cells to anticancer drugs. To clarify whether ABCG2 inhibition is involved in the sensitizing effect of celecoxib, we investigated whether the expression of ABCG2 in breast cancer cell lines ...

Background: Currently, ARTs are of indispensable importance in treatment of human infertility. While ARTs are considered to be safe, some studies suggest that ARTs manipulations may perturb the epigenetic information and subsequently the health of the offspring. To examine these events further, this study was conducted to assess the effect of superovulation, in vitro culture and vitrification o...