Monoxenic cultures of burrowing nematode populations extracted from banana roots from Belize, Guatemala, Honduras, and Costa Rica were established on carrot discs. Cultures of Radopholus spp. were also obtained from Florida, Puerto Rico, Dominican Republic, and Ivory Coast. The aggressiveness (defined as reproductive fitness and root necrosis) of these populations was evaluated by inoculating b...

When studying digestibility, the respective parts of the exogenous, endogenous and bacterial fractions in the digesta or feces must be measured. The proportions of proteins from different sources may be estimated by comparing their amino acid composition with those of reference sources. This study describes the composition of endogenous and microbial proteins, i.e. meconium of piglet small inte...

Genetic exchange between cells of Trypanosoma brucei that occurs in the tsetse fly vector (Jenni et al. 1986) is an important part of the life cycle that contributes significantly to genetic diversity of these human pathogens (Gibson and Stevens 1999). Indirect observations have also indicated the presence of sexuality during the development of Trypanosoma cruzi (Bogliolo et al. 1996; Stothard ...

A simple, rapid, and inexpensive method for evaluation of host-parasite interactions, based on monoxenic cultures, is described. Axenic root explants of Glycine max (L.) Merr., cultured on a holidic agar medium, were inoculated with axenic second-stage larvae of Heterodera glycines Ichinohe, Race 3. A clear separation of susceptible and resistant cultivars, based on numbers of mature female nem...

When surface-sterilized spores of the arbuscular mycorrhizal fungus (AMF) Glomus intraradices Sy167 were germinated on agar plates in the slightly modified minimum mineral medium described by G. Bécard and J. A. Fortin (New Phytol. 108:211-218, 1988), slime-forming bacteria, identified as Paenibacillus validus, frequently grew up. These bacteria were able to support growth of the fungus on the ...

Endosymbiont-bearing trypanosomatids have been considered excellent models for the study of cell evolution because the host protozoan co-evolves with an intracellular bacterium in a mutualistic relationship. Such protozoa inhabit a single invertebrate host during their entire life cycle and exhibit special characteristics that group them in a particular phylogenetic cluster of the Trypanosomati...

The reproductive fitness of seven isolates of Pratylenchus vulnus from different geographical areas and hosts was assessed in monoxenic cultures (carrot), and greenhouse cultures (plum, sour orange, and quince). The genetic makeup of the different isolates was compared by Random Amplified Polymorphic DNA (RAPD-PCR). The apple (PvAP-S) and apricot (PvAT-F) isolates reproduced less in monoxenic c...