The present study was undertaken to investigate the effect of diacerein on the histopathology of articular cartilage and subchondral bone of the femorotibial joint in rats. Osteoarthritis was induced in rats after single intra-articular injection of sodium iodoacetate. Rats were sacrificed 1, 2, 4, and 8 weeks post intra-articular injection to evaluate the progression of histopathogenesis of os...

The style component of the self-incompatibility (S) locus of the wild tomato Lycopersicon peruvianum (L.) Mill. is an allelic series of glycoproteins with ribonuclease activity (S-RNases). Treatment of the S3-RNase from L. peruvianum with iodoacetate at pH 6.1 led to a loss of RNase activity. In the presence of a competitive inhibitor, guanosine 3'-monophosphate (3'-GMP), the rate of RNase inac...

Volume regulation of C6 glial cells was studied in anoxia in vitro to improve the understanding of ischemic cell swelling in the brain. Contrary to in vivo conditions, anoxia or anoxia plus iodoacetate for additional inhibition of anaerobic energy metabolism did not induce glial swelling. However, intracellular K+ was markedly decreased while intracellular Na+ increased. Induction of energy fai...

The style component of the self-incompatibility (S) locus of the wild tomato Lycopersicon peruvianum (L.) Mill. is an allelic series of glycoproteins with ribonuclease activity (S-RNases). Treatment of the S,-RNase from L . peruvianum with iodoacetate at p H 6.1 led to a loss of RNase activity. In the presence of a competitive inhibitor, guanosine 3”Ionophosphate (3‘-CMP), the rate of RNase ina...

has been crystallized from Can&&z utilis (1). The kinetics (2) and the stereochemistry of the reaction (3) have been studied, but very little is known of the nature of the functional groups or amino acids involved in the catalysis. This report deals with the role of one or two sulfhydryl groups in the activity of the enzyme. Rapid inactivation has been found to take place upon treatment with io...

The active cysteine of papain was labeled with W-iodoacetate and the cystine residues were reduced and coupled with unlabeled iodoacetate. The heptacarboxymethyl papain was then maleylated and hydrolyzed with trypsin. Key peptides were isolated from this hydrolysate which have permitted completion of the amino acid sequence of the protein. Thus, an earlier tentative and incomplete version of th...

A lipoxygenase inhibitor in the cytosolic fraction of human epidermoid carcinoma A431 cells was characterized and purified. The cytosolic inhibitor lost the inhibitory activity upon heating at 75 degrees C for 15 min or pretreating with 1 mg/ml trypsin at 37 degrees C for 60 min. Cytosol, after dialysis, lost the inhibitory activity but its inhibitory activity recovered when 1 mM GSH was added ...

When oxidized to cysteic acid by performic acid or converted to carboxymethylcysteine by alkylation of the reduced enzyme with iodoacetate, a total of six half-cystine residues/subunit are found in L-threonine dehydrogenase (L-threonine: NAD+ oxidoreductase, EC 1.1.1.103; L-threonine + NAD(+)----2-amino-3-oxobutyrate + NADH) from Escherichia coli K-12. Of this total, two exist in disulfide link...