the present study utilizes an analytical method to formulate the three lowest modal frequencies of axially-loaded notched beam through both crack location and load level in a specific format that can be used in existing frequency-based crack-identification methods. the proposed formula provides a basis to shift into two states, one with axial loading and the other without any loading whatsoever...

Despite its prevalence and physical importance in biology, the mechanical properties of molecular collagen are far from established. The flexibility of the triple helix is unresolved, with descriptions from different experimental techniques ranging from flexible to semirigid. Furthermore, it is unknown how collagen type (homo- vs. heterotrimeric) and source (tissue-derived vs. recombinant) infl...

Polar residues lying between adjacent α-helical chains of coiled-coils often contribute to coiled-coil curvature and flexibility, while more typical core hydrophobic residues anneal the chains together. In tropomyosins, ranging from smooth and skeletal muscle to cytoplasmic isoforms, a highly conserved Asp at residue 137 places negative charges within the tropomyosin coiled-coil core in a posit...

This paper introduces a set of sensitivity metrics to be used along likelihood-based modal identification methods. In maximum likelihood (ML) estimation theory, the precision of ML point estimates can be measured by the curvature of the likelihood function. This paper presents closed-form partial derivatives, observed information, and variance expressions for discrete-time stochastic state-spac...

β-lactamases are bacterial enzymes that confer resistance to β-lactam antibiotics, such as penicillins and cephalosporins. There are four classes of β-lactamase enzymes, each with characteristic sequence and structure properties. Enzymes from class A are the most common and have been well characterized across the family; however, less is known about how physicochemical properties vary across th...

This chapter describes marketing strategies in concept for wireless broadband services in the Sri Lankan market. It also emphasizes different technologies offering fixed and mobile broadband services. Wi-Fi services which are mentioned here has been on offer for few years but actual marketing of such services are not actively done in Sri Lanka. Various marketing strategies that could be used to...

The flexibility and curvature of duplex DNAs containing mismatched sites have been monitored as a function of temperature. The diffusion coefficients are dependent on the flexibility and the curvature of the DNA, and these have been determined by NMR-based methods. The diffusion coefficients, D, depend on a Boltzmann term and the viscosity of the solvent, eta, which is also temperature dependen...

We have developed a high-throughput approach to the labor-intensive problems of DNA cyclization, which we use to characterize DNA curvature and mechanical properties. The method includes a combinatorial approach to make the DNA constructs needed and automated real-time measurement of the kinetics using fluorescence. We validated the approach and investigated the flexibility of two kinds of nick...