Streptococcus milleri NMSCC 061 produces an endopeptidase, millericin B, which hydrolyzes the peptide moiety of susceptible cell wall peptidoglycan. The nucleotide sequence of a 4.9-kb chromosomal region showed three open reading frames (ORFs) and a putative tRNA(Leu) sequence. The three ORFs encode a millericin B preprotein (MilB), a putative immunity protein (MilF), and a putative transporter...

Since the discovery of ammonia-oxidizing archaea (AOA), new questions have arisen about population and community dynamics and potential interactions between AOA and ammonia-oxidizing bacteria (AOB). We investigated the effects of long-term fertilization on AOA and AOB in the Great Sippewissett Marsh, Falmouth, MA, USA to address some of these questions. Sediment samples were collected from low ...

During the Subarctic Pacific Iron Experiment for the Ecosystem Dynamics Study (SEEDS), chlorophyll a concentration in the iron patch reached near maximum in 9 days (D9) after the iron fertilization and after that remained more or less constant. In spite of the stability of chlorophyll a, primary production was higher than 1.4–2.0 g C m d during D9–D13. As most of the carbon synthesized by phyto...

on the proportion of colour-marked offspring. Labelling of living spermatozoa with fluorescein isothiocyanate was used to establish the identity of spermatozoa recovered from the female tract and/or associated with eggs following mixed inseminations. Little difference in the proportion of the two sperm populations present in the female tract was apparent 6 hr after insemination but, by 13 hr, t...

The effect of a new feed additive “GLINMOL” in the form emulsion on productivity sturgeons was studied. aim research to study newly developed additives based fish milt roe and glycerin sturgeon productivity. experiment consisted two parts; groups 3-4 4 maturity stages were formed. first group (control) received complete mixed without additives, second - 2.0% total weight; third at dose weight. ...

The objective of this study was to evaluate the importance of environmental and genetic factors affecting semen quality; sperm volume (SV), sperm concentration (SPCO), total sperm (TS), fresh sperm motility (FSM), total fresh motile sperm (TFMS), post thaw sperm motility (PTSM), total post thaw motile sperm (TPTMS), number of produced payout (NPP), number of fresh motile sperm in each milt ejac...

Ammonia oxidation by microorganisms is a rate-limiting step of the nitrification process and determines the efficiency of fertilizer utilized by crops. Little is known about the dynamic response of ammonia-oxidizers to different fertilization regimes in a wheat-rice rotation system. Here, we examined ammonia-oxidizing bacteria (AOB) and archaea (AOA) communities across eight representative stag...

High inbreeding depression is thought to be one of the major factors preventing evolutionary transitions in hermaphroditic plants from self-incompatibility (SI) and outcrossing toward self-compatibility (SC) and selfing. However, when selfing does evolve, inbreeding depression can be quickly purged, allowing the evolution of complete self-fertilization. In contrast, populations that show interm...

Chest tubes are utilized to evacuate shed blood after left ventricular assist device (LVAD) implantation, however, they can become clogged, leading to retained blood. We implemented a protocol for active tube clearance (ATC) of chest tubes to determine if this might reduce interventions for retained blood. A total of 252 patients underwent LVAD implantation. Seventy-seven patients had conventio...

Recent studies found fish egg-specific bacterial communities that changed over the course of embryogenesis, suggesting an interaction between the developing host and its microbiota. Indeed, single-strain infections demonstrated that the virulence of opportunistic bacteria is influenced by environmental factors and host immune genes. However, the interplay between a fish embryo host and its micr...