In this paper we describe the microcomputer-aided determination of cell proliferation kinetics and doubling times utilizing a crystal violet assay and a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide assay in microtitration plates. The analysis of spectrophotometric data provides the doubling times at any time of incubation. Plots of doubling time versus time of incubation giv...

Minimum inhibitory and bactericidal concentrations of four antibiotics and their combinations were determined for 38 strains of enterococci by a microtitration tube dilution technique. The drugs were ampicillin, vancomycin, gentamicin, and streptomycin; the combinations were ampicillin-gentamicin, ampicillin-streptomycin, vancomycin-gentamicin, and vancomycin-streptomycin. At achievable serum c...

HERR, S., TE BRUGGE, LESLEY A. & GUINEY, M. C. M., 1982. The value of the microtitre serum agglutination test as a second screening test in bovine brucellosis. Oruierstepoort Journal of Veterinary Research, 49, 23--28 (1982). The use of the serum agglutination test (SAT) as a 2nd screening test led to a reduction in false negative screening reactions especially in sera delayed in transit and pr...

The relationship between antibody titres in the microtitration serum agglutination test and the complement fixation test in bovine brucellosis is described. For low and high MSAT values there is good agreement between the 2 tests. This is not the case for MSAT values between 54 and 338 IU/ml. For practical reasons, results falling into this category cannot all be repeated. Repetitions are so st...

BACKGROUND Potency test for control of rubella vaccine is a significant factor to qualify production line and vaccination program. For this reason, WHO recommends to use the microtitration method by both vaccine companies and control laboratories. Then the study was done to improve this test. METHODS Three rubella virus samples, including an in-house standard, a lot of vaccine and an in-proce...

This paper presents a convenient micromethod for the estimation of amino acids, based on the reaction with ninhydrin. The ammonia formed in this reaction is determined, after elimination of the interference from ninhydrin with superoxol, by addition of saturated potassium hydroxide, aeration, and titration with standard acid. The application of the reaction with ninhydrin is one of the most spe...

BACKGROUND The extreme specific activity of the long-lifetime fluorescent europium(III) chelate nanoparticles and the enhanced monovalent binding affinity of multivalent nanoparticle-antibody bioconjugates are attractive for noncompetitive immunoassay. METHODS We used a noncompetitive, two-step immunoassay design to measure free prostate-specific antigen (PSA). Europium(III) chelate nanoparti...

This study was designed to evaluate the cytotoxic effect of copper nanoparticles in murine adenocarcinoma cells (AMN-3). The exposure period cell line performed at 24 hr a microtitration plate under complete sterile conditions. Different concentrations were used started from 0.39 μgmL-1to 50 μgmL-1 three independent experiments. First, stained by MTT and absorbance measured using Elisa reader...