Somatic DNA alterations are known to occur in atherosclerotic carotid artery lesions; however, their significance is unknown. The accumulation of microsatellite mutations in coding DNA regions may reflect a deficiency of the DNA mismatch repair (MMR) system. Alternatively, accumulation of these coding microsatellite mutations may indicate that they contribute to the pathology. To discriminate b...

UNLABELLED PREMISE OF THE STUDY The exploitation of Labisia pumila for commercial demand is gradually increasing. It is therefore important that conservation is prioritized to ensure sustainable utilization. We developed microsatellites for L. pumila var. alata and evaluated their polymorphism across var. alata, var. pumila, and var. lanceolata. • METHODS AND RESULTS Ten polymorphic micros...

The Antarctic toothfish, Dissostichus mawsoni , serves as a valuable fishery resource around the Continent since 1997, managed by Commission for Conservation of Marine Living Resources (CCAMLR). Although delineating genetic or stock structure populations is crucial improving management this species, its number and diversity levels remain ambiguous. In present study, we assessed population phylo...

It has been 20 years since the first development of DNA fingerprinting and the start of forensic DNA typing. Ever since, human tandem repeat DNA sequences have been the main targets for forensic DNA analysis. These repeat sequences are classified into minisatellites (or VNTRs) and microsatellites (or STRs). In this brief review, we discuss the historical and current forensic applications of suc...

We have screened the nearly complete DNA sequence of the human Y chromosome for microsatellites (short tandem repeats) that meet the criteria of having a repeat-unit size of > or = 3 and a repeat count of > or = 8 and thus are likely to be easy to genotype accurately and to be polymorphic. Candidate loci were tested in silico for novelty and for probable Y specificity, and then they were tested...

In the context of this paper microsatellites (short approximate tandem repeats) refer to consecutive patterns contained in genomic sequences. A new algorithm to detect such microsatellites in DNA is proposed. The algorithm relies on the construction of finite automata originating from the Moore machine paradigm. The proposed finite automata contain “counting states”. The overall algorithm is de...

The construction of high-density linkage maps for use in identifying loci underlying important traits requires the development of large numbers of polymorphic genetic markers spanning the entire genome at regularly spaced intervals. As part of our efforts to develop markers for rainbow trout (Oncorhynchus mykiss), we performed a comparison of allelic variation between microsatellite markers dev...

Source/Description: Clones containing [CA]n microsatellites were selected with end-labelled [TG])5 from a flow-sorted chromosome 9 library (derived from a human/hamster hybrid cell line) in Charon 21 A. Southern blots of clones containing microsatellites were probed with either human or hamster DNA. Clone 9CMP1 containing a [CA]n microsatellite of human origin was sequenced enabling the design ...