Species. Oncorhynchus mykiss. Source and Description. Microsatellite repeats were identified by sequencing clones from microsatellite-enriched libraries constructed from genomic DNA. Microsatellite markers were developed by designing primers to amplify the repeats. Primer Sequences. See Table 1. Method of Detection. PCR conditions were optimized using rainbow trout DNA (Kamloop strain) and mini...

Microsatellite loci (also known as simple sequence repeats [SSRs] or simple tandem repeats [STRs]) are regions of DNA that contain short (<6 bp) tandemly repeated sequences (Litt and Luty 1989; Edwards et al. 199 1; Jacob et al. 199 1). Much attention is currently being paid to the development of microsatellite loci because they display a number of properties of interest for genomic mapping, po...

The human genome is laden with both non-LTR (long-terminal repeat) retrotransposons and microsatellite repeats. Both types of sequences are able to, either actively or passively, mutagenize the genomes of human individuals and are therefore poised to dynamically alter the human genomic landscape across generations. Non-LTR retrotransposons, such as L1 and Alu, are a major source of new microsat...

To date we have little knowledge of how accurate next-generation sequencing (NGS) technologies are in sequencing repetitive sequences beyond known limitations to accurately sequence homopolymers. Only a handful of previous reports have evaluated the potential of NGS for sequencing short tandem repeats (microsatellites) and no empirical study has compared and evaluated the performance of more th...

Pea aphids represent a complex genetic system that could be used for QTL analysis, genetic diversity and population genetics studies. Here, we described the development of first microsatellite repeat database of the pea aphid (APMicroDB), accessible at "http://deepaklab.com/aphidmicrodb". We identified 3,40,233 SSRs using MIcroSAtellite (MISA) tool that was distributed in 14,067 (out of 23,924)...

Microsatellites appear widely in genomes of diverse species. Variants of repeat number of microsatellites often correlate with risks of genetic disorder or severity of diseases. Using cross-species comparison, the proposed system comprehensively verifies microsatellites of specific genes related to 16 genetic disorders. Genomic information retrieved from 14 frequently used model organisms in bi...

BACKGROUND Microsatellite instability (MSI) is a useful phenotype in cancer diagnosis and prognosis. Nevertheless, methods to detect MSI status from next generation DNA sequencing (NGS) data are underdeveloped. METHODS We developed an approach to detect the MSI phenotype using NGS (mSINGS). The method was used to evaluate mononucleotide microsatellite loci that were incidentally sequenced aft...

UNLABELLED PREMISE OF THE STUDY Microsatellite markers were developed from a New Zealand endemic understory tree, Fuchsia excorticata, to investigate factors affecting the mating system. • METHODS AND RESULTS Using 454 pyrosequencing, 48 microsatellite markers were developed and tested for polymorphism within populations. Twelve of these microsatellite loci were identified as being polymor...