The advent of next-generation sequencing (NGS) technologies has transformed the way microsatellites are isolated for ecological and evolutionary investigations. Recent attempts to employ NGS for microsatellite discovery have used the 454, Illumina, and Ion Torrent platforms, but other methods including single-molecule real-time DNA sequencing (Pacific Biosciences or PacBio) remain viable altern...

Background. The aim of this study was to determine the correlation between MSI and sporadic colorectal cancer in Guilan province, North part of Iran. Materials and Methods. A total of 96 patients who underwent resection for sporadic colorectal cancer in Guilan province were studied. No patients had positive family history of cancers. The frequencies of MSI were analyzed by testing the BAT-26 an...

JAK1 frameshift mutations may promote cancer cell immune evasion by impeding upregulation of the antigen presentation pathway in microsatellite unstable endometrial cancers (ECs). This study investigated the JAK1 mutation frequency, its functional implication in immune evasion and its prognostic significance in microsatellite unstable EC. Microsatellite instability and three microsatellite repe...

INTRODUCTION The transcription factor Fli1 is implicated in the pathogenesis of systemic lupus erythematosus (SLE). Recently, a GA(n) polymorphic microsatellite was characterized in the mouse Fli1 promoter that modulates promoter activity and is truncated in two lupus mouse models compared to non-autoimmune prone mice. In this work, we characterize a homologous GA(n) microsatellite in the human...

The azimuthal version of the magnetorotational instability (MRI) is a nonaxisymmetric instability of a hydrodynamically stable differentially rotating flow under the influence of a purely or predominantly azimuthal magnetic field. It may be of considerable importance for destabilizing accretion disks, and plays a central role in the concept of the MRI dynamo. We report the results of a liquid m...

Fifteen microsatellite loci were studied in Drosophila melanogaster and Drosophila simulans, two closely related sibling species which split 2-3.5 MYA. Within-species variances in repeat number were found to differ up to 1,000-fold among individual microsatellite loci. A significant correlation of log variances between both species indicated a locus-specific mutation rate of microsatellites. He...

The development of microsatellite DNA markers for use with butterflies and moths is extremely difficult for no apparent reason. New findings demonstrate that many lepidopteran microsatellite sequences exist in multiple copies in the genome, and have similar or almost identical flanking regions. These findings provide a compelling explanation for the low efficiency of microsatellite isolation in...

Microsatellite analysis is an important tool in clinical research and molecular diagnostics because microsatellite instability (MSI) occurs frequently in various types of cancer. Approximately 10-15% of colorectal, gastric and endometrial carcinomas are associated with MSI, and this has an impact on clinical prognosis. The microsatellite loci Bat25, Bat26, D2S123, D5S346 and D17S250, recommende...

The aims of the study were to detect the polymorphisms of three microsatellite sites: S11, S15, and S24 in the ovine PRNP and to asses their relationship with prion protein (PrP) genotypes in three sheep breeds. To identify 15 PrP genotypes based on polymorphisms at codons 136, 154, and 171, PCR-RFLP analyses were applied. The microsatellite sites were amplified. For each microsatellite two or ...

the aim of the present study was to find the best or optimum topology of six chicken populations, which were genotyped based on nine highly polymorphic microsatellite markers. to reach this goal, different genetic distances based on infinite allele model (iam), stepwise mutation model (smm) and drawing phylogentic trees on un-weighted pair-group method using arithmetic averages (upgma) method w...