The use of poly(N-vinyl-2-pyrrolidone) (PVP) hydrogel-supported luminol chemiluminescence (CL) for the automatic determination of hydrogen peroxide and the quantification of the antiradical capacity of Trolox is described. The hydrogel containing luminol and hemin is prepared directly on a 96-well microplate and can be stored for up to 3 months without significant decrease in CL quantum yields....

A rapid colorimetric, microplate based nitrate reductase assay (NRA) method for minimum inhibitory concentration (MIC) determination of clinical isolates of Mycobacterium tuberculosis was compared with the microplate Alamar Blue assay (MABA), presently in vogue. Reproducible results were obtained in 7 days by NRA. The NRA method was found to be inexpensive, suitable for MIC determination agains...

Equipment and additionally required solutions The -Gal Reporter Gene Assay can be performed in all automated or manual luminometers in tube and microplate format, as well as in scintillation counters. When using the microplate format, black or white microplates must be used. Except for redist. water (required for reconstitution and dilution purposes), the kit contains all assay reagents for lys...

Seven hundred thirty-two female urogenital samples were collected for Chlamydia trachomatis testing by both the Sanofi Diagnostics Pasteur (Chaska, Minn.) Chlamydia Microplate EIA by the shortened protocol and the Syva (San Jose, Calif.) MicroTrak II EIA, and the results were compared with those obtained by cell culture. For the analysis of samples from female patients, the patients were divide...

BACKGROUND Urinary iodine is a good biochemical marker for control of iodine deficiency disorders. Our aim was to develop and validate a simple, rapid, and quantitative method based on the Sandell-Kolthoff reaction, incorporating both the reaction and the digestion process into a microplate format. METHODS Using a specially designed sealing cassette to prevent loss of vapor and cross-contamin...

Evaluation of renal hemodynamics requires estimation of effective renal plasma flow, which is commonly measured by the renal clearance of p-aminohippuric acid (PAH). There are many existing methods for PAH assay that are complicated, expensive, or time consuming. We describe a rapid, precise, and accurate microplate-based assay of PAH using p-dimethylaminocinnamaldehyde, which produces a red co...

There is a need for accurate, high-throughput, functional measures to gauge the efficacy of potential drugs in living cells. As an early marker of drug response in cells, cellular metabolism provides an attractive platform for high-throughput drug testing. Optical techniques can noninvasively monitor NADH and FAD, two autofluorescent metabolic coenzymes. The autofluorescent redox ratio, defined...

Chlamydomonas reinhardtii (Cr), a unicellular alga, is routinely utilized to study photosynthetic biochemistry, ciliary motility, and cellular reproduction. Its minimal culture requirements, unicellular morphology, and ease of transformation have made it a popular model system. Despite its relatively slow doubling time, compared with many bacteria, it is an ideal eukaryotic system for microplat...

Reproductive toxicity of 2-bromopropane (2BP), a substitute for ozone layer-depleting chloro-fluorocarbon, was found among the workers in an electronics factory in Korea in 1995. Furthermore the importance of testicular toxicity has been realized since the problem of endocrine disruptors arose all over the world, but manual methods must rely on subjective assessment. Recently, computer-assisted...

The TopCountTM Microplate Scintillation Counter quantitates radioisotopes by liquid scintillation counting in standard microplate formats. Assays that previously had to be transferred from microplates to vials or to non-standard formats for counting can now be quantitated directly in microplates. TopCount can count up to 12 samples simultaneously with performance comparable to that of discrete ...