Micronuclei frequency, a marker of genotoxicity, was studied within a trial of alpha-tocopherol for chemoprevention of oral leukoplakia. Oral swabs were obtained from two sites, the leukoplakia lesion and normal-appearing mucosa, at baseline and following 24 weeks of therapy with 400 international units of alpha-tocopherol twice daily. These specimens were analyzed for micronuclei frequency. Th...

BACKGROUND Cigarette smoke has been identified as the main cause of oral cavity carcinoma. Recently, the electronic cigarette, a battery-operated device, was developed to help smokers stop their tobacco addiction. This study aimed to evaluate the safety of electronic cigarettes and to establish the possible role of such device in the primary prevention of oral cavity cancer. SUBJECTS AND METH...

In the present study, the combination of the micronucleus test with analysis of the activity of the rRNA genes in mutagen-treated Hordeum vulgare (barley) by maleic hydrazide (MH) cells was performed. Simultaneously fluorescence in situ hybridization (FISH) with 25S rDNA as probes and an analysis of the transcriptional activity of 35S rRNA genes with silver staining were performed. The results ...

Glyphosate is a widely used broad-spectrum weed control agent. In the present study, an in vivo study on the genotoxic effects of a technical herbicide (Roundup) containing isopropylamine salt of glyphosate was carried out on freshwater goldfish Carassius auratus. The fish were exposed to three doses of glyphosate formulation (5, 10 and 15 ppm). Cyclophosphamide at a single dose of 5 mg/l was u...

Biomarkers for increased cytogenetic damage in smokers include sister chromatid exchanges (SCE) in peripheral lymphocytes and micronuclei in sputum cells. These markers may reflect increased cancer risk. Increased cancer risk has also been associated with lower blood levels of the antioxidants beta-carotene and vitamin C and with genetic deficiency of the detoxification enzyme glutathione S-tra...

OBJECTIVE The aim of this study was to assess the incidence of micronuclei (MN) in buccal mucosal cells of professionals working in radiology area to determine the risk of stochastic effects of radiation. MATERIALS AND METHODS All the professionals and students working in King Khalid University - College of Dentistry radiology area were included in the Risk Group (RG = 27). The Control Group ...

The frequency of micronuclei in bone marrow cells of mice treated or not with Liv. 52 and then exposed to 4.5 Gy of gamma-radiation was evaluated from 6 h to 14 days post irradiation. The frequency of micronuclei increased from 6 h to 24 h post irradiated in both irradiated groups and declined thereafter, the frequency of micronuclei remaining significantly lower in the Liv. 52-treated group. T...

The ability of two calix[4]arene derivatives, namely 25,27-p-tert-butylcalix[4]dithiooxabenzocrown (1) and 25,27-p-tert-butylcalix[4]trithiooxabenzocrown (2), to produce chromosomal aberrations in root meristematic cells of Allium cepa and micronuclei (MN) in normochromatic erythrocytes (NCE) of Balb/c mice was investigated. NCE are normal mature red blood cells with a full complement of hemogl...

methotrexate is an antineopalstic agent widely used in low dose to treat patients with rheumatoid arthritis. it is known to induce micronuclei at multiple doses in rats. the present study investigates the effect of vitamin a on methotrexate-induced micronuclei in rat bone marrow erythrocytes. male wistar rats were (n=5) injected with 0, 8, 16 and20mg/kg methotrexate (single i.p dose). a group o...