The spread of multidrug-resistant Enterobacteriaceae related to the production of extended-spectrum β-lactamases and carbapenemases is a serious public health problem worldwide. Microbiological diagnosis and therapy of these infections are challenging and controversial. Clinically relevant questions were selected and the literature was reviewed for each of them. The information from the selecte...

Mapping DNase-I hypersensitive sites (HS) was used in the past to identify regulatory elements of specific genes. More recently, thousands of HS were identified in the human genome by using high-throughput methods. These approaches showed a general enrichment of HS near or within known genes, within CpG islands, within human-mouse conserved regions and in GC-rich regions of the genome. Here we ...

OBJECTIVE To evaluate the prevalence of methicillin resistant Staphylococcus aureus (MRSA) carriage among a cohort of pilgrims during 2004 Hajj season. METHODS Pilgrims attending the 2004 Hajj season were recruited and screened for carriage of MRSA. Standard microbiological techniques were used to screen for the presence of MRSA. RESULTS Out of 411 individuals screened, 85 (20.6%) were posi...

Capturing microbial growth on a macroscopic scale is of great importance to further our understanding of microbial life. However, methods for imaging microbial life on a scale of millimeters to centimeters are often limited by designs that have poor environmental control, resulting in dehydration of the agar plate within just a few days. Here, we created MOCHA (microbial chamber), a simple but ...

Fosfomycin has shown promising in vitro activity against multidrug-resistant (MDR) urinary pathogens; however, clinical data are lacking. We conducted a retrospective chart review to describe the microbiological and clinical outcomes of urinary tract infections (UTIs) with MDR pathogens treated with fosfomycin tromethamine. Charts for 41 hospitalized patients with a urine culture for an MDR pat...

Multi-drug therapy is the standard-of-care treatment for tuberculosis. Despite this, virtually all studies of the pharmacodynamics (PD) of mycobacterial drugs employed for the design of treatment protocols are restricted to single agents. In this report, mathematical models and in vitro experiments with Mycobacterium marinum and five antimycobacterial drugs are used to quantitatively evaluate t...

This research aims to evaluate the application of GMP and SSOP systems determine quality jambal roti salted fish produced at Mamah Jambal MSMEs, Pangandaran. The was conducted from September 2021 January 2022 using case study method. procedure included observation processing stages, prerequisite program deviation analysis, microbiological, chemical, metal contamination testing. data obtained co...

Local-structure scattering refinements using a simultaneous fit of x-ray/neutron total scattering, EXAFS, and diffuse scattering in electron diffraction are implemented as an extension to the RMCProfile software Version 6. Users should refer to the main RMCProfile manual for this version for details regarding RMC refinements using total scattering data alone. A user is expected to have a reason...

Astronomical phenomena are governed by processes on all spatial and temporal scales, ranging from days to the age of the Universe (13.8 Gyr) as well as from kilometre size up to the size of the Universe. This enormous range in scales is contrived, but as long as there is a physical connection between the smallest and largest scales it is important to be able to resolve them all, and for the stu...

Spliceosomal intron numbers and boundary sequences vary dramatically in eukaryotes. We found a striking correspondence between low intron number and strong sequence conservation of 5' splice sites (5'ss) across eukaryotic genomes. The phylogenetic pattern suggests that ancestral 5'ss were relatively weakly conserved, but that some lineages independently underwent both major intron loss and 5'ss...