An enzyme catalyzing the synthesis of L-cystathionine from the succinyl ester of L-homoserine and L-cysteine was isolated from a Salmonella mutant blocked in another step of methionine synthesis, and derepressed for cystathionine y-synthetase formation. The approximate molecular weight of the enzyme was 155,000 and it was unchanged after removal of tightly bound pyridoxal phosphate. In the abse...

A new product (Application 460B-00) from Agilent Technologies/WassonECE uses a 5973N GC/MSD (gas chromatograph/mass selective detector) for the determination of trace levels of impurities that are moderate-to-low carbon-content such as oxygenates, mercaptans, sulfides, arsine, and phosphine in ethylene and propylene. This work describes the performance of Application 460B-00 with respect to lin...

A. Equilibrium and second-order rate constants have been measured for the redox reaction between phthaloquinone and dihydroriboflavin (and its reverse) at a number of pH values. The equilibria and kinetic determinations are in agreement. No intermediates could be found in the reaction, and it is proposed that with this and other quinones electron transfer occurs within a complex of the flavin a...

Compounds containing sulfhydryl groups have occupied a unique position in metabolic and chemical studies because of their intimate association with the problems of protein structure (l), enzyme function (2), and cellular proliferation (3). Kolthoff and Harris (4) described an amperometric method for the determination of mercaptans which Benesch and Benesch adapted for use with amino acids and p...

Five sulfur-containing flavor compounds were synthesized for the first time by the reaction of 4-ethyloctanoyl chloride with sulfur-containing alcohols or mercaptans. The synthesized compounds are 3-(methylthio)propyl 4-ethyloctanoate, 2-methyl-3-tetrahydro-furanthiol 4-ethyloctanoate, 4-methyl-5-thiazoleethanol 4-ethyloctanoate, 2-furan-methanethiol 4-ethyloctanoate and 2-methyl-3-furanthiol 4...