The objective of this study was to evaluate genetic stability by determining the meiotic index (MI) and pollen viability in F1RC2 segregating populations, obtained crosses between wheat commercial cultivars synthetic wheat, aimed at introgression/genetic resistance. For MI, three ears were collected per genotype, before anthesis, they fixed Carnoy’s solution. cytological slides prepared crushin...

Budding yeast Pch2 protein is a widely conserved meiosis-specific protein whose role is implicated in the control of formation and displacement of meiotic crossover events. In contrast to previous studies where the function of Pch2 was implicated in the steps after meiotic double-strand breaks (DSBs) are formed, we present evidence that Pch2 is involved in meiotic DSB formation, the initiation ...

....................................................................................................................... vi Chapter 1: Introduction .............................................................................................. 1 The switch between the mitotic and meiotic transcriptome ................................ 2 Regulatory processing of meiotic transcripts ...................

<i>Carex brevispicula</i> (Cyperaceae) is endemic to Korea and characterized by constricted achenes, short lateral spikes, awned staminate pistillate scales. The species classified in sect. <i>Mitratae</i> occurs throughout South Korea, perennating on mountains and/or rocky slopes under half shadow conditions. Meiotic chromosomes of the were examined this study, which 33...

Ski8p is implicated in degradation of non-poly(A) and double-stranded RNA, and in meiotic DNA recombination. We have identified the Sordaria macrospora SKI8 gene. Ski8p is cytoplasmically localized in all vegetative and sexual cycle cells, and is nuclear localized, specifically in early-mid-meiotic prophase, in temporal correlation with Spo11p, the meiotic double-strand break (DSB) transesteras...

Although in Saccharomyces cerevisiae the initiation of meiotic recombination, as indicated by double-strand break formation, appears to be functionally linked to the initiation of synapsis, meiotic chromosome synapsis in Drosophila females occurs in the absence of meiotic exchange. Electron microscopy of oocytes from females homozygous for either of two meiotic mutants (mei-W68 and mei-P22), wh...

Meiotic exchange occurs preferentially between homologous chromatids, in contrast to mitotic recombination, which occurs primarily between sister chromatids. To identify functions that direct meiotic recombination events to homologues, we screened for mutants exhibiting an increase in meiotic unequal sister-chromatid recombination (SCR). The msc (meiotic sister-chromatid recombination) mutants ...

Abstract Study question Can Interluekin-6 (IL-6) induce spermatogonial cells from seminiferous tubules of normal or busulfan-treated mice to develop spermatogenesis in vitro using methylcellulose culture system (MCS)? Summary answer IL-6 induced the development premeiotic, meiotic and post-meiotic vitro. What is known already Spermatogenesis a complicated process sperm generation. During this p...

Timing and duration of pre-meiotic G1, S and G2 phases in Paramecium caudatum have been clarified by microspectrophotometry of DNA content and autoradiography with [3H]thymidine ([3H]dThd) and [3H]deoxyuridinemonophosphate ([3H]dUMP). Microspectrophotometric measurement of DNA content showed that cells in stationary phase have a G1 micronucleus, and that pre-meiotic DNA synthesis begins with th...

In eukaryotes, diploid cells give rise to haploid cells via meiosis, a program of two cell divisions preceded by one round of DNA replication. Although key molecular components of the meiotic apparatus are highly conserved among eukaryotes, the mechanisms responsible for initiating the meiotic program have diverged substantially among eukaryotes. This raises a related question in animals with t...