Optimization of production medium is required to maximize the metabolite yield. This can be achieved by using a wide range of techniques from classical "one-factor-at-a-time" to modern statistical and mathematical techniques, viz. artificial neural network (ANN), genetic algorithm (GA) etc. Every technique comes with its own advantages and disadvantages, and despite drawbacks some techniques ar...

A new approach using microtiter plate cultivation with on-line measurement of dissolved oxygen (DO) was applied for medium optimization of mammalian cell culture. Applying dynamic liquid phase balance, oxygen uptake rates were calculated from the DO level and used as an indicator for culture viability. The developed method was successfully applied to optimization of the concentration of glucose...

In this study, Bacillus licheniformis cells were immobilized by entrapment in calcium alginate beads and were used for production of alkaline protease by repeated batch process. In order to increase the stability of the beads, the immobilization procedure was optimized by statistical full factorial method, by which three factors including alginate type, calcium chloride concentration, and agita...

It is now widely accepted that calcium (Ca) deficiency is linked to potato (Solanum tuberosum L.) tuber disorders and improved tuber health is expected through increased Ca availability. The purpose of this study was to optimize the Ca concentration in Murashige-Skoog (MS) basal medium for microtuber production and determine microtuber mineral composition, especially Ca content. The response of...

conclusions optimization of medium composition and synergistic antimicrobial activity on the cyanobacteria could lead to production of effective antimicrobials. results c. leptolyngbya had antimicrobial activity individually and in synergy with antibiotics against gram-positive and gram-negative bacteria. the highest activity of the chloroform extract was on gram positive strains and its effect...