Establishment of apical-basal polarity is crucial for epithelial sheets that form a compartment in the body, which function to maintain the environment in the compartment. Effects of impaired polarization are easily observed in three-dimensional (3-D) culture systems rather than in two-dimensional (2-D) culture systems. Although the mechanisms for establishing the polarity are not completely un...

The paper presents the results of a study of cultural characteristics of the strain A/equine/Otar/764/07 (H3N8) of equine influenza virus isolated from sick horse during the outbreak of equine influenza epizooty in the Republic of Kazakhstan. The studies determined the optimal culture conditions for equine influenza virus strain A/equine/Otar/764/07 (H3N8) in MDCK cell culture. The optimal infe...

MDCK cells in culture form a functional transporting epithelium. Apical surface of MDCK monolayers are not adhesive to free MDCK cells that fail to grow attached to existing monolayers. In contrast, when plated at high density (106 cm-2) MDCK cells form multilayered structures in which only the outermost layer shows a typical apical surface (microvilli, tight junctions, etc). In the lower layer...

Madin-Darby canine kidney (MDCK) cells undergoing cystogenesis in vitro is a scientifically useful model of epithelial morphogenesis. The cysts formed in collagen and Matrigel are qualitatively similar, consisting of a single layer of epithelial cells surrounding a hollow lumen. However, differences in key quantitative measures of cyst growth, including cell number and cyst and lumen size, indi...

PURPOSE To characterize the response of cultured human retinal pigment epithelial (RPE) cells to lowered environmental oxygen. METHODS The response of cultured RPE cells to lowered oxygen environments was compared to that of cell types of presumed high (Madin-Darby canine kidney [MDCK] cells, an epithelial cell line) and low (CSF, corneal stromal fibroblasts) aerobic requirements. Cultures in...

STUDY OBJECTIVE To evaluate P-glycoprotein (P-gp)-mediated renal drug interactions in an in vitro model of tubular secretion. DESIGN In vitro experiment. SETTING University-affiliated pharmacokinetics laboratory. CELL LINES: Madin-Darby canine kidney (MDCK), multidrug-resistant-1 (MDR1)-MDCK, and human colon carcinoma (Caco-2) cells. INTERVENTION Transepithelial transport (basolateral-to-...

Thiazolidinediones have been reported to retard cystic disease in rodent models by uncertain mechanisms. We hypothesized that their major effect in retarding cystogenesis was through inhibiting cell proliferation or stimulating apoptosis. In the Madin-Darby canine kidney cell (MDCK) model, rosiglitazone inhibited cyst growth in a time- and dose-dependent manner and this was accompanied by a red...

In cell culture a kidney epithelial cell line MDCK, forms a continuous sheet of identically oriented asymmetrical cells joined by circumferential occluding junctions. The reconstructed epithelial membrane has transport and permeability qualities of in vivo transporting epithelia. The cell layer can be readily manipulated when cultured on a freely permeable membrane filter and, when placed in an...

Seasonal influenza epidemics occur both in northern and southern hemispheres every year. Despite the differences virus surface antigens virulence of seasonal subtypes, manufacturers are well-adapted to respond this periodical vaccine demand. Due decades research, development new vaccines is relatively straight forward. In similarity with ongoing coronavirus disease 2019 pandemic, manufacturing ...