Acute promyelocytic leukemia (APL) is a well-defined entity among acute leukemia, cytogenetically characterized by a t(15;17) (q22;q11-12) translocation. In vitro and in vivo studies suggest that all-trans retinoic acid (RA) treatment restores cell maturation. We have isolated the first permanent cell line with t(15;17), derived from the marrow of a patient with APL in relapse. The establishmen...

Background This study aimed to investigate whether Artificial Seminiferous Tubules (AST) coated with testicular extra cellular matrix (ECM) support maturation of spermatogenic cells from immature testes. MaterialsAndMethods Medical grade tubes coated with testicular ECM were used. Testes from immature mice were loaded in the concentration of 10000- 20000/ml. Tubes were then immersed in germ cel...

introduction: effects of immediate tnf-α exposure on phenotype and function of dendritic cells (dcs) derived from cord blood mono nuclear cells materials and methods: umbilical cord blood mncs were isolated from healthy mothers and were divided into tnf(+( and tnf (-) groups. both were cultured using scf, flt3l, gm-csf and il-4. but, three ng/ml of tnf-α was first added in the culture of tnf(+(...

Sertoli cells undergo a maturation process during post-natal testicular development that leads to the adult-type Sertoli cell, which is required for spermatogenesis. Understanding Sertoli cell maturation is therefore necessary to gain insight into the underlying causes of impaired spermatogenesis and male infertility. The present study characterized the cellular and molecular differentiation of...

BACKGROUND Dendritic cell (DC) defects may contribute to chronicity in hepatitis C virus (HCV) infection and determine response to PEG-interferon and ribavirin therapy via poor T cell stimulation. Studies to date have produced inconsistent results regarding DC maturation and function: no large study has examined DCs before and after therapy. AIMS We examined if DC defects in maturation and ch...

An individual's immune function, susceptibility to infection, and response to immunosuppressive therapy are influenced in part by his/her T cell maturation state. Although childhood is the most dynamic period of immune maturation, scant information regarding the variability of T cell maturation in children with renal disease is available. In this study, we compared the T cell phenotype in child...

CONTEXT Maturation delay of germ cells and their progression into carcinoma in situ (CIS) frequently occurs in intersex patients. A developmentally delayed germ cell resembles a CIS cell and displays prolonged expression of immunohistochemical markers used for the diagnosis of CIS. This questions their applicability in young children. OBJECTIVE The objective of the study was the elaboration o...

To analyze the function of AP-1 in cartilage formation, two types of primary chondrocytes, LS and US cells, were prepared from caudal (lower) and cephalic (upper) regions of chicken sterna, respectively. All the known components of chicken AP-1 (c-Fos, Fra-2, c-Jun, and JunD) were detected in both cell types, but the expression level of c-Jun was much higher in LS cells, which are rich in less ...