The present study aimed evaluate an on-farm culture system for identification of milk pathogens associated with clinical mastitis in dairy cows using two different gold standard approaches: standard laboratory culture in study 1 and 16S rRNA sequencing in study 2. In study 1, milk from mastitic quarters (i.e. presence of flakes, clots, or serous milk; n = 538) was cultured on-farm using a singl...

A single intramammary infusion of recombinant bovine interleukin-8 (IL-8) at 50 μg/quarter/head, but not 10 μg/quarter/head, induced clinical mastitis in three of four cows during the dry-off period, resulting in an elevated rectal temperature, redness and swelling of the mammary gland, extensive polymorphonuclear leukocyte (PMNL) infiltration, and milk clot formation from 1 to 28 days post inf...

The aims of this study were to isolate and identify of methicillin resistant Staphylococcus aureus (MRSA) from mastitic cow milk. The investigation presence of mecA genes and molecular characteristics of these isolates using eight primers of repetitive element palindromic-PCR (Rep-PCR) analysis were achieved. Ninety six milk samples, collected from clinical mastitic cows from Sharkia Governorat...

14 15 In this paper the implementation of a tool was considered, based on Machine Learning (ML) techniques to improve 16 the detection of subclinical mastitis in dairy herds. The analysed population included 71 dairy farms in Asturias 17 (Northern Spain) and a total of 2,407 lactating cows. Four sources of information were available: a) a questionnaire 18 survey describing facilities, milking r...

Lysozyme activity in buffalo milk in relation to the period of lactation, parity of animal, weather conditions and udder infections was studied. Effect of storage and heat processing of milk on lysozyme activity was determined. Lysozyme activity was higher in buffalo milk than in cow milk. Buffalo colostrum showed lysozyme activity 5 times of that in mature milk. Lysozyme activity in buffalo mi...

Bovine mastitis is a disease that concerns animals' welfare and increases the economic production losses. Bacterial agents such as Staphylococcus species are main causative agent of bovine mastitis. This bacterial expresses some inflammatory cytokines might enhance cell-mediated, which may promote pathogenesis The objective current study was to investigate innate immune response circulating lev...

The present study was conducted to investigate the effect of subclinical mastitis on clinicopathological changes in Mastitic friesian. A total of 400 individual milk samples from clinically normal udder quarters of 100 diary friesians were examined microbiologically as well as by using California mastitis test (C.M.T.) for detection of subclinical mastitis and designing rapid diagnostic tests f...

A cross sectional study was carried out from November 2016 to April 2017 in and around Asella town to determine the prevalence, associated risk factors and antimicrobial susceptibility of Staphylococcus aureus in Bovine mastitic milk. A total of 384 lactating dairy cows were screened for mastitis based on clinical examinations and California Mastitis Test (CMT). Out of 230 lactating crossbred c...