Bacterial halo blight (BHB) is a bacterial disease, caused by Pseudomonas syringae pv. garcae, which has been gaining prominence in the main coffee-producing regions. Chemical control of this disease increases production costs and environmentally undesirable. In scenario, development new cultivars resistant to BHB most economical sustainable alternative. Marker-Assisted Selection (MAS) an appro...

Objectives: There are some controversies about the value of modified Ashworth Scale (MAS) for assessing spasticity. The goal of this study was to investigate if there is any correlation between scores obtained from MAS for wrist spasticity and electrophysiological recordings as the objective measure of spasticity. Methods: In this cross-sectional study, 34 stroke patients were employed. Wris...

The random amplified polymorphic DNA (RAPD) technique has been used widely for purposes such as the construction of linkage maps, QTL analysis, evaluation of genetic diversity, and parentage tests. However, some minor bands of RAPD display a low reproducibility and low reliability. We describe a simple and efficient method for making minor RAPD bands clearer. The first step of this method is to...

ferent alternatives for defining a strategy. The screened population size has been reported in simulation studies Designing a highly efficient backcross (BC) marker-assisted selecas the most important factor affecting the efficiency of tion (MAS) experiment is not a straightforward exercise, efficiency being defined here as the ratio between the resources that need to MAS (Zhang and Smith, 1992...

We describe a simple multiplex allele-specific (MAS)-PCR assay to detect mutations in the second base of the katG gene codon 315, including AGC-->ACC and ACA (Ser-->Thr) substitutions that confer resistance to isoniazid (INH) in Mycobacterium tuberculosis clinical isolates. The 315 ACC allele is found in the majority of Inh(r) strains worldwide, especially in areas with a high incidence of tube...

Common bacterial blight (CBB) caused by Xanthomonas campestris pv. phaseoli and X. campestris pv. phaseoli var. fuscans is a seed-borne disease that can signifi cantly reduce yield and seed quality of common bean (Phaseolus vulgaris L.). Disease resistance is the most effective management strategy, but most common bean cultivars have no CBB resistance. Marker-assisted selection (MAS) shows prom...

There are currently different techniques, such as CGH arrays, to study genetic variations in patients. CGH arrays analyze gains and losses in different regions in the chromosome. Regions with gains or losses in pathologies are important for selecting relevant genes or CNVs (copy-number variations) associated with the variations detected within chromosomes. Information corresponding to mutations...