Highly purified murine granulocyte-macrophage progenitor cells (CFU-GM) were used as target cells to assess the possible direct effects of purified preparations of recombinant murine gamma-interferon, prostaglandin E, recombinant human heavy chain (acidic) ferritin, and recombinant human tumor necrosis factor alpha (TNF-alpha) on progenitor cells in vitro. Target CFU-GM, with cloning efficienci...

Myeloid cell lines and primary leukemic myeloblasts express two classes of granulocyte macrophage colony-stimulating factor (GM-CSF) binding sites of high (Kd 20-50 pM) and low affinity (Kd 5-10 nM). High affinity binding is caused by the association of two chains, p80 alpha and p130 beta, whereas p80 alpha alone confers low affinity binding only. Furthermore interleukin-3 (IL-3) competes for t...

The macrophage-specific colony-stimulating factor (CSF-1, M-CSF) regulates the survival, growth and differentiation of monocytes. We have recently demonstrated that phorbol ester induces expression of CSF-1 in human monocytes. These findings suggested that activated monocytes are capable of producing their own lineage-specific CSF. The present studies demonstrate that the granulocyte-macrophage...

Hematopoietic stem/progenitor cells (HSPCs) which give rise to different blood cell types are present within the bone marrow microenvironment, especially in flat bones such as skull, vertebrae, pelvis and chest. Interacting factors such as stromal derived factor-1/CXCR4, very late antigen-4/vascular cell adhesion molecule-1, Lymphocyte function-associated antigen-1/ intercellular adhesion molec...

Anti-murine (m) interleukin-5 (IL-5) antibody was found to inhibit eosinophil (Eo) colony formation stimulated by recombinant human (rh) IL-5, but did not inhibit the production of Eo stimulated by rh lL-3 or granulocyte-macrophage colony-stimulating factor (GM-CSF). Conditioned medium (CM) prepared from eosinophilic patients’ T cells with interleukin-2 (IL-2) stimulation (T-lL-2-CM), was found...

We compared the radiosensitivity of macrophage (M) colony-forming cell (CFC) in and outside the hemopoietic bone marrow. Murine bone marrow cells (BMC) are stimulated by either macrophage colony-stimulating factor (CSF-1) or murine recombinant granulocyte-macrophage colony-stimulating factor (rGM-CSF) to form M- and granulocyte- macrophage (GM) colonies on soft agarose medium, whereas both peri...