AIMS Different cultural conditions for forming and reverting protoplasts were systematically studied to establish a rapid and efficient protocol for Sclerotium rolfsii ATCC 201126. METHODS AND RESULTS Osmotic stabilizer, lytic enzymes and mycelial age were the main factors influencing protoplast yields. An optimized protocol involving 1-h hydrolysis of 45-h-old mycelium with Trichoderma harzi...

BACKGROUND Reduced yields of ethanol due to bacterial contamination in fermentation cultures weaken the economics of biofuel production. Lactic acid bacteria are considered the most problematic, and surveys of commercial fuel ethanol facilities have found that species of Lactobacillus are predominant. Bacteriophage lytic enzymes are peptidoglycan hydrolases that can degrade the Gram positive ce...

The negative impact of chemical pesticides on the environment and human health has contributed to introduction legal regulations that ensure reduction in use agrochemicals favor biological products. existing review literature, including our research, clearly shows ideal biocontrol agents are Trichoderma fungi. production antibiotics, lytic enzymes degrading cell walls plant pathogens, or induci...

OBJECTIVES In an innovative therapeutic exploitation against antibiotic-resistant Streptococcus pneumoniae, here we have evaluated the in vitro activity of a purified bacterially-encoded cell wall lytic enzyme, LytA (the major pneumococcal autolysin), and compared it with those of Cpl-1 and Pal (pneumococcal phage lytic enzymes) and two antibiotics versus four pneumococcal strains. METHODS Tw...

Lysobacter species have attracted increasing attention in recent years due to their capacities produce diverse secondary metabolites against phytopathogens. In this research, we analyzed the genomic and transcriptomic patterns of capsici CK09. Our data showed that L. CK09 harbored various contact-independent biocontrol traits, such as fungal cell wall lytic enzymes HSAF/WAP-8294A2 biosynthesis,...