Abstract—Lyophilization, also called freeze-drying, is an important dehydration technique mainly used for pharmaceuticals. Food industry also uses lyophilization when it is important to retain most of the nutritional quality, taste, shape and size of dried products and to extend their shelf life. Vacuum-Induced during freezing cycle (VI) has been used in order to control ice nucleation and, con...

OBJECTIVE The preservation of Streptococcus pneumoniae by standard freezing methods for subsequent tests--such as serotyping and antibiotic susceptibility--is not possible or is difficult in many developing countries because of the high cost of equipment, inadequate equipment maintenance, and irregular power supply. We evaluated alternative low-cost methods, by comparing different culture media...

Storage of cultures of mycobacteria by freezing in skimmed milk was determined to be an easier and more reliable method of maintaining viability and stability than lyophilization.

conclusions although quince seeds dried mucilage by lyophilization had less yield amounts yet in view of purity and physicochemical and mechanical properties, it was significantly better than mucilage prepared by the oven method (p < 0.05). results the yield value of mucilage from the warm method was about three folds greater than the amount from the cold method. the phytochemical, physicochemi...

The application of lyophilization to the preservation of BCG, in an effort to combat the rapid loss in allergenic potency of the liquid vaccine, has presented important technical difficulties. Although the effectiveness of lyophilized BCG in producing tuberculin conversion appears to be as great as that of the fresh vaccine,"118,2 clinical experience has shown that its use engenders quantitativ...

PURPOSE The major limitation in the widespread use of liposome drug delivery system is its instability. Lyophilization is a promising approach to ensure the long-term stability of liposomes. The aim of this study was to prepare sirolimus-loaded liposomes, study their stability and investigate the effect of lyophilization either in the presence or in the absence of lyoprotectant on liposome prop...

Influenza virosomes are reconstituted influenza virus envelopes that may be used as vaccines or as carrier systems for cellular delivery of therapeutic molecules. Here we present a procedure to generate influenza virosomes as a stable dry-powder formulation by freeze-drying (lyophilization) using an amorphous inulin matrix as a stabilizer. In the presence of inulin the structural integrity and ...

The ability to detect nuclear damage is an important tool for the development of sperm preservation methods. We used the acridine orange test (AOT) and the terminal deoxynucleotidyl transferase-mediated dUDP nick-end labeling (TUNEL) assay to assess the DNA status of sperm cells preserved with different lyophilization media. The AOT did not detect any differences between different lyophilizatio...