The pattern of inhibition and recovery of intracellular Sadenosylhomocysteine hydrolase (AdoHcyase) activity in duced by cytotoxic concentrations of 2'-deoxyadenosine (dAdo) in the presence of the adenosine deaminase inhibitor erythro-9-[3-(2-hydroxynonyl)]adenine was studied in cultured human T-lymphoblasts (CCRF-CEM and -HSB), Epstein-Barr virus-transformed B-lymphoblasts (JP and RDG), and re...

Abstract Chronic myeloid leukaemia most commonly presents in chronic phase (CML-CP) and it is characterised by granulocytic proliferation. Many patients have an excellent response to tyrosine kinase inhibitor therapy; however, a small proportion will develop lymphoid or blast crisis, with inferior clinical outcomes. Detection of lymphoblasts at diagnosis CML-CP has been reported case series con...

Human lymphoblasts in long-term culture are specifically agglutinated by concentrations of concanavalin A that do not agglutinate normal human lymphocytes. The agglutination is inhibited by a-D-methyl mannoside. Lymphoblast lines kept in long-term culture bound 2 to 3 times more concanavalin A per cell than did normal lymphocytes. Normal lymphocytes and lymphoblasts bound approximately equivale...

Heteroantisera were raised in rabbits against human thymocytes or Burkitt's lymphoma EBI cells. After suitable absorption, the sera were specific for thymocytes and T lymphoblasts, or B lymphoblasts and several lymphoblastoid cell lines. The specificity of the antisera was confirmed by preparing purified populations of T or B lymphocytes on immunoabsorbent columns, followed by mitogenic stimula...

Azurophilic granulation of blasts is a feature of acute myeloid leukemia (AML). Granular acute lymphoblastic leukemia (ALL) may mimic AML due to the presence of cytoplasmic granules in lymphoblasts, but cytochemistry and immunophenotyping are helpful in making the correct diagnosis. Toluidine blue (TB) is a metachromatic dye, which stains basophils and myeloid blasts that exhibit basophilic dif...

Herpesvirus (GPHV) antigen is either present in very small amounts, or absent in leukemic lymphoblasts taken directly from strain 2 guinea pigs. However, after maintenance in tissue culture for 72 hr, almost 100% of these lymphoblasts contained GPHV antigen. The expression of GPHV antigen could be demonstrated by indirect immunofluorescent technique as well as by the direct (125)I labeled antib...

The stimulation of lymphocyte transformation by autologous human lymphoblasts established in continuous culture has been reported by several laboratories (1-6). The nature of the determinants on cultured lymphoblasts that stimulate transformation of autologous lymphocytes is not clear, but may be related to what appears to be a universal infection (latent or expressed) of all lymphoblast lines ...

A previous publication documented a sarcomatous transformation observed in cell line derived from rat lymphoma growing monolayer culture. (9) The morphological and cytogenetic changes found, suggested the presence of virus differentiation malignant lymphoblasts toward histiocytes present paper describes, under electron microscope structural features original tumor, cells culture sarcoma. This s...

The presence of lymphocyte receptors for peanut agglutinin in significant numbers (greater than 15%) was identified on leukemic cells from T-cell acute lymphoblastic leukemia (T-ALL) (3/4), B-cell ALL (B-ALL) (2/4), null cell ALL (8/17), and on normal fetal thymic lymphocytes but not on normal human peripheral blood lymphocytes. Peanut agglutinin (PNA) binding was blocked specifically on leukem...

The status of three purine pathway enzymes, adenosine deaminase, 5'-nucleotidase, and purine nucleoside phosphorylase, was evaluated in the leukemic cells of patients with acute lymphoblastic leukemia and correlated with routine immunological cell surface markers. A distinct pattern of enzyme activity was noted in T-lymphoblasts which have significantly higher adenosine deaminase activity (p < ...