نتایج جستجو برای: luminometer
تعداد نتایج: 171 فیلتر نتایج به سال:
Special calorimeters are needed to instrument the very forward region of an ILC detector. These devices will improve the hermeticity being important for new particle searches. A luminometer is foreseen to measure the rate of low angle Bhabha scattering with a precision better than 10. A calorimeter adjacent to the beam-pipe will be hit by a large amount of beamstrahlung remnants. The amount and...
Ozone is a triatomic allotropic modification of oxygen with very high oxidation potential and strong antimicrobial properties, can be used as disinfecting agent. The aim this work was to investigate the effectiveness gaseous ozone in reducing number bacteria total biomass E. coli biofilm using different methods quantification detection. Biofilm all tested clinical isolates standard strain grown...
A chemiluminescence immunoassay (CLI) for the direct measurement of aldosterone in serum was developed with aminobutylethyl isoluminol (ABEI) as the label. In this competitive assay the samples are incubated with sample, antibody, aldosterone-carboxymethyl oxime-ABEI, and paramagnetic particles coated with second antibody. After magnetic separation and washing, the samples are incubated with 20...
We report a gold nanoparticle (AuNP)-capped mesoporous silica nanoparticle (Au-MSN) platform for intracellular codelivery of an enzyme and a substrate with retention of bioactivity. As a proof-of-concept demonstration, Au-MSNs are shown to release luciferin from the interior pores of MSN upon AuNP uncapping in response to disulfide-reducing antioxidants and codeliver bioactive luciferase from t...
Natural proteins can be used in measuring intracellular Ca(2+) concentration. As one of the Ca(2+)- regulated photoproteins, aequorin has several advantages in comparison to widely used Ca(2+) fluorescence indicators (e.g., fura-2, indo-1 and fluo-3), including high dynamic range and resistance to motion artefacts. However, incorporation of aequorin into cells remains a challenge. Hypoosmotic s...
We evaluated the power of pregnant women's neutrophils in releasing the oxidant substances (such as H202, superoxide, ...) in the presence of luminol. In the presence of luminol these substances will emit light and we measured this light by a luminometer (chemiluminescence technicque). Baker's yeast was used for neutrophil activation.This study includes 45 pregnant women (mean age = 30.7 years)...
Microbial fuel cells (MFCs) offer an excellent solution to tackle some of the major challenges currently faced by humankind: sustainable energy sources, waste management and water stress. Besides treating wastewater producing useful electricity from urine, ceramic MFCs can also generate biocidal catholyte in-situ. It has been proved that generation a high impact in composition. Therefore, compo...
The LEP ee collider at CERN operated for more than a decade: in 198995 at center of mass energies close to the Z peak (LEP-I); in 1996-2000 at higher energies, up to 209 GeV (LEP-II). In the first phase a large number of Z events were collected, of the order of 5 × 10 events per experiment. To match the inherent precision of this data sample, the error on the integrated luminosity had to be bet...
The use of poly(N-vinyl-2-pyrrolidone) (PVP) hydrogel-supported luminol chemiluminescence (CL) for the automatic determination of hydrogen peroxide and the quantification of the antiradical capacity of Trolox is described. The hydrogel containing luminol and hemin is prepared directly on a 96-well microplate and can be stored for up to 3 months without significant decrease in CL quantum yields....
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