99S macrophage represented at least one important source of BAL IL-8 in the patients who subsequently developed ARDS. In established ARDS, both neutrophil chemotaxins were elevated together with MCP-1, but not MIP-ia. These data suggest that different chemotactic cytokines are employed at different stages of the evolution of ARDS. BAL IL-8 may represent a very early marker of the inflammatory r...

OBJECTIVES The aim of the present study was to investigate the effects of lipopolysaccharide (LPS) on the migration and adhesion of human dental pulp stem cells (hDPSCs) and the associated intracellular signalling pathways. METHODS hDPSCs obtained from impacted third molars were exposed to LPS and in vitro cell adhesion and migration were evaluated. The effects of LPS on gene expression of ad...

the inhibition of the inducible nitric oxide synthase (inos), cyclooxygenase 2 (cox-2) and nuclear factor-κb (nf-κb) production are research targets of attract in the field of anti-inflammatory drug development. therefore, this study was designed to investigate the anti-inflammatory effects of novel thiazolidinone derivatives using a cellular model of lipopolysaccharide (lps)-stimulated murine ...

BACKGROUND Exposure to subclinical levels of lipopolysaccharide (LPS) occurs commonly and is seemingly well tolerated. However, recurrent LPS exposure induces cardiac fibrosis over 2 to 3 months in a murine model, not mediated by the renin-angiotensin system. Subclinical LPS induces cardiac fibrosis by unique mechanisms. METHODS In C57/Bl6 mice, LPS (10 mg/kg) or saline (control) were injecte...

A three-dimensional (3D) dentin/pulp tissue analogue, resembling the human natural has been engineered in an vitro setup, aiming to assess cytocompatibility of resin-based dental restorative cements. Stem Cells from Apical Papilla (SCAP) and Human Umbilical Vein Endothelial (HUVEC) were embedded Collagen-I/Fibrin hydrogels at 1:3 ratio within 24-well plates. Hanging culture inserts placed over ...

Aim: To investigate the effects of propofol on toll-like receptor 4 (TLR4) signaling pathway induced by lipopolysaccharide (LPS) in alveolar macrophages (AMs). Methods: AMs isolated from 10 patients were cultured for 1 hour. These cells were pooled and randomly divided into five groups. The final concentrations of LPS and propofol were 1μg/mL and 30μM, respectively. AMs were continually culture...

Melatonin, which is the main product of the pineal gland, has well documented antioxidant and immune-modulatory effects. Macrophages produce molecules that are known to play roles in inflammatory responses. We conducted microarray analysis to evaluate the global gene expression profiles in response to treatment with melatonin in lipopolysaccharide (LPS) activated RAW 264.7 macrophage cells. In ...

Purpose: To detect the effect of Resolvin D1 (RvD1) on Developmental endothelial locus-1 (Del-1) and cytokine expressions human gingival fibroblast cells exposed to Porphyromonas gingivalis lipopolysaccharide (P. gingivalis-LPS).
 
 Material Methods: The RvD1 cell viability fibroblasts P. gingivalis-LPS were determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromi...

Background: Methyl jasmonate is a bioactive oxylipid that participates in the defense-related mechanisms of plants. The anti-inflammatory and anti-oxidative capacities methyl against lipopolysaccharide (LPS) induced arthritis have been widely investigated. However, role LPS-induced cell model tubular-interstitial nephritis (TIN) has not reported. Methods: LPS (5 µg/mL) was applied to treat huma...

Periodontitis is a frequent chronic inflammatory disorder destroying periodontium. Recent studies have revealed the role of bromodomain and extraterminal domain inhibitor (BETi) microRNA (miR)-130a in regulating macrophage polarization pro-inflammatory response. However, little known about whether apabetalone (a novel BETi) miR-130a are correlated with state periodontitis by polarization. Here ...