A quantum mechanical (QM) method rooted on density functional theory (DFT) has been employed to determine conformations of the methane-thiosulfonate spin label (MTSL) attached to a fragment extracted from the activation loop of Aurora-A kinase. The features of the calculated energy surface revealed low energy barriers between isoenergetic minima, and the system could be described in a populatio...

Membrane encapsulation is frequently used by the cell to sequester biomolecules and compartmentalize their function. Cells also concentrate molecules into phase-separated protein or protein/nucleic acid "membraneless organelles" that regulate a host of biochemical processes. Here, we use solution NMR spectroscopy to study phase-separated droplets formed from the intrinsically disordered N-termi...

Endothelial lipase (LIPG) and zinc finger protein 202 (ZNF202) are two pivotal genes in high density lipoprotein (HDL metabolism). We sought to determine their genetic contribution to variation in HDL-cholesterol levels by comprehensive resequencing of both genes in 235 individuals with high or low HDL-C levels. The selected subjects were 141 Whites (High HDL Group: n = 68, [Formula: see text] ...

The effective treatment of cow milk allergy in infants consists of elimination of cow milk protein and the introduction of formulas based on an extensively hydrolyzed protein formula or an amino acid-based formula. However, about 10% of these infants are still allergic to an extensively hydrolyzed protein formula and an amino acid-based formula is very expensive. We conducted a study to verify ...

Twenty eight lactating buffaloes were used in a completely randomized design with 2×2 factorial arrangement of four experimental diets including low protein–low energy (LP-LE), low protein–high energy (LP-HE), high protein–low energy (HP-LE) and high protein–high energy (HP-HE). Results showed that the HP-HE diet recorded the highest digestibility coefficients of CP, EE, NFE, nutritive values, ...

twenty eight lactating buffaloes were used in a completely randomized design with 2×2 factorial arrangement of four experimental diets including low protein–low energy (lp-le), low protein–high energy (lp-he), high protein–low energy (hp-le) and high protein–high energy (hp-he). results showed that the hp-he diet recorded the highest digestibility coefficients of cp, ee, nfe, nutritive values, ...

iii