Articles about the development of derivative spectrophotometric methods and analytical applications of derivative spectrophotometry (DS) published in the last nine years (since 1994) are reviewed.

A spectrophotometric method was developed for simultaneous determination of amlodipine (Aml) and valsartan (Val) without previous separation. In this method amlodipine in methanolic solution was determined using zero order UV spectrophotometry by measuring its absorbency at 360.5 nm without any interference from valsartan.Valsartan spectrum in zero order is totally overlapped with that of amlod...

We have previously shown that an association of losartan and hydrochlorothiazide, initiated 1 mo after 5/6 nephrectomy (Nx), reversed hypertension and albuminuria and promoted lasting renoprotection. In this new study, we investigated whether equal or even better protection could be obtained by combining losartan and furosemide. Nx was performed in 58 Munich-Wistar rats. One month later, tail-c...

Micellar high-performance liquid chromatography (HPLC) and first-derivative ultraviolet spectrophotometry were used to simultaneously determine fluconazole (FLZ) and tinidazole (TNZ) in combined pharmaceutical dosage forms. The derivative procedure is based on the linear relationship between the drug concentration and the first derivative amplitudes at 220 and 288 nm for FLZ and TNZ, respective...

Objective: The present study was designed with an objective of a simple, fast, precise, selective and accurate RP-HPLC method was developed and validated for the simultaneous determination of Losartan, Hydrochlorothiazide and Amlodipine from bulk and formulations. Methods: Reverse phase high performance liquid chromatography (RP-HPLC) method was developed and validated for rapid analysis of sim...

In this study, a derivative method is applied for the determination of dye concentration in fabrics dyed with tricomponent dye mixtures. The reflectance of spectra of the samples were recorded between 400 and 700 nm. Initially, the obtained (K/S) spectra were divided by the spectrum of a standard sample dyed with two of the components in the ternary mixture and the derivative spectra were calcu...

1-(2-Pyridylazo)-2-naphthol (PAN) has been used for the simultaneous and individual determination of palladium and cobalt at trace levels. PAN complexes of palladium and cobalt at neutral pH form green-color neutral complexes, which are soluble in aqueous SDS micellar media. Under optimum conditions, calibration graphs for individual determinations by zero and first-derivative spectrophotometry...

in iran, the majority of vegetable oil producers , use pet for packaging their products . due to high solubility of pet oligomers in the oils , and shelf life of liquid oils , it is expected that some compounds migrate into the oil . this study was carried out to determine the amount of migration of terephtalates from pet bottles into sunflower oil and the effect of temperature and storage time...

Two simple, accurate and reproducible spectrophotometric methods have been developed for the simultaneous estimation of Hydrochlorothiazide (Hctz), Atenolol (Atn) and Losartan potassium (Los) in combined tablet dosage forms. The first method involves determination using the simultaneous equation method, the sampling wavelengths selected are, 272.5 nm, 224 nm and 250 nm over the concentration ra...