نتایج جستجو برای: live tissues

تعداد نتایج: 289137  

Journal: :PLoS ONE 2009
Nikos Kourtis Nektarios Tavernarakis

Analysis of general and specific protein synthesis provides important information, relevant to cellular physiology and function. However, existing methodologies, involving metabolic labelling by incorporation of radioactive amino acids into nascent polypeptides, cannot be applied to monitor protein synthesis in specific cells or tissues, in live specimens. We have developed a novel approach for...

2015
Gražvydas Lukinavičius Claudia Blaukopf Elias Pershagen Alberto Schena Luc Reymond Emmanuel Derivery Marcos Gonzalez-Gaitan Elisa D'Este Stefan W. Hell Daniel Wolfram Gerlich Kai Johnsson

Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utility. Here we describe a far-red DNA stain, SiR-Hoechst, which displays minimal toxicity, is applicable in different cell types and tissues, and...

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