A total of 46 Listeria spp. were isolated from 719 samples (milk, bulk tank swabs, cheese, feed, water, faeces and the environment) collected from 415 cattle and 304 sheep over 12 months (from February 2007 to January 2008). These isolates were identified by conventional and PCR techniques as belonging to L. monocytogenes (17.4%), to Listeria innocua (39.1%), to Listeria seeligeri 17.4%), to Li...

Previous studies have shown that early phagosome-endosome fusion events following phagocytosis of Listeria monocytogenes are modulated by the live organism. In the present study, we have characterized more fully the intracellular pathway of dead and live Listeria phagosomes. To examine access of endosomal and lysosomal markers to phagosomes containing live and dead Listeria, quantitative electr...

The innate immune system senses pathogens by pattern recognition receptors in different cell compartments. In the endosome, bacteria are generally recognized by TLRs; facultative intracellular bacteria such as Listeria, however, can escape the endosome. Once in the cytosol, they become accessible to cytosolic pattern recognition receptors, which recognize components of the bacterial cell wall, ...

Listeria monocytogenes is a ubiquitous food-borne pathogen, and its presence in food or production facilities highlights the importance of surveillance. Increased understanding of the surface exposed antigens on Listeria would provide potential diagnostic and therapeutic targets. In the present work, using mass spectrometry and genetic cloning, we show that fructose-1,6-bisphosphate aldolase (F...

introduction: different microbial compounds can induece maturation of dendritic cells (dcs) into dc1 or dc2 .in the present study, we used tumor antigen pulsed dendritic cell that matured with listeria monocytogenes antigen and cholera toxin (ct) in the treatment of a murin model of cancer. material and methods: tumor cells (wehi-164 balb/c derived fibrosarsoma) were injected subcutaneously to ...

Infection due to Listeria monocytogenes mostly effects immunocompromized hosts (1,2). Although, viruses usually cause encephalitis and bacterial infections are associated with meningeal inflammation, Listeria monocytogenes is one of the few bacterial microorganisms that may present as a meningoencephalitis and most frequently as a rhombencephalitis (1,2). Listeria rhombencephalitis causes respi...

Immunity against infection with Listeria monocytogenes is not achieved from innate immune stimulation by contact with killed but requires viable Listeria gaining access to the cytosol of infected cells. It has remained ill-defined how such immune sensing of live Listeria occurs. Here, we report that efficient cytosolic immune sensing requires access of nucleic acids derived from live Listeria t...

Immunization of mice with nonviable Listeria monocytogenes generates an insufficient CD8(+) T cell response and consequently only limited protection against subsequent L. monocytogenes infection. We have recently demonstrated that depletion of regulatory CD4(+) T cells during immunization significantly enhances CD8(+) T cell responses. In the present study, we determined the impact of CD4(+) T ...

Listeriosis in human is clinically characterized as perinatal listeriosis, neonatal listeriosis and adult listeriosis. Listeria spp are an established pathogen in animals, and human infections are usually seen following ingestion of contaminated food (processed food). Human infections of Listeria spp initially present with gastrointestinal symptoms. Meningitis, septicaemia, brain abscess and pr...

discussion l. monocytogenes can cause progressive and rapidly fatal infections in the vulnerable persons, with multisystem involvement. since this bacterium is not susceptible to cephalosporines, it will be better to consider effective antimicrobials in the treatment of the possible cases. case presentation a 70-years-old patient with chronic renal failure was admitted to the infectious disease...