Although the literature contains many studies on the function of UCP3, its role is still being debated. It has been hypothesized that UCP3 may mediate lipid hydroperoxide (LOOH) translocation across the mitochondrial inner membrane (MIM), thus protecting the mitochondrial matrix from this very aggressive molecule. However, no experiments on mitochondria have provided evidence in support of this...

Lipid and protein oxidation are well-known manifestations of free radical activity and oxidative stress. The current study investigated extermination of Leishmania tropica promastigotes induced by lipid and protein oxidation with reactive oxygen species produced by PEGylated metal-based nanoparticles. The synthesized photodynamic therapy-based doped and nondoped zinc oxide nanoparticles were ac...

INTRODUCTION Obesity is an important risk factor for atherosclerotic cardiovascular disease. Paraoxonase-1 (PON1) may play a significant role in the prevention of obesity-related accelerated atherosclerosis by hydrolyzing lipid peroxides in oxidized low-density lipoproteins. OBJECTIVES The aim of this study was to evaluate PON1 and arylesterase enzyme activities and lipid hydroperoxide (LOOH)...

PURPOSE To measure endothelial function and oxidative stress immediately, 90 minutes, and three hours after exercise of varying intensities. METHODS Sixteen apparently healthy men completed three exercise bouts of treadmill running for 30 minutes at 55% V̇O(2max) (mild); 20 minutes at 75% V̇O(2max) (moderate); or 5 minutes at 100% V̇O(2max) (maximal) in random order. Brachial artery flow-mediate...

OBJECTIVE Paraoxonase is a high density lipoprotein-associated anti-oxidant enzyme. The aim of this study was to investigate the serum paraoxonase and arylesterase activities and associations between these enzymes and lipid hydroperoxide (LOOH) in preeclamptic patients. MATERIALS AND METHODS The study included 47 preeclamptic (25 severe and 22 mild preeclampsia cases) and 20 normotensive heal...

Introduction. Ruptured abdominal aortic aneurysm (rAAA) causes a significant inflammatory response. The study aims to investigate this response following endovascular and open repair of ruptured AAA. Patients and Methods. Consecutive rAAA patients had either endovascular aneurysm repair (EVAR) or open repair (OR). Blood samples were taken for cytokines, lipid hydroperoxides (LOOH), antioxidants...

Oxidative modification of low density lipoprotein (LDL) has been suggested as a causal step in atherosclerosis, and both redox-active transition metal ions and superoxide (O2.-) have been implicated in this process. In order to determine the mechanisms of metal ion-dependent oxidation of LDL in the presence of O2.-, LDL was exposed to hypoxanthine (HX) and purified xanthine oxidase (XO) without...

UNLABELLED The relationship between mesh weight and host tissue reaction has, so far, not been fully investigated. Lightweight meshes (LWM) are thought to give less inflammatory response compared with heavyweight meshes (HWM). The present study is a randomized, controlled, double-blind clinical trial performed in 61 patients who underwent an elective inguinal hernioplasty. The primary outcome o...

Hydroperoxides are the primary oxygenated products of polyunsaturated fatty acids and were determined spectrophotometrically based on their reaction with an excess of Fe2+ at low pH in the presence of the dye Xylenol Orange. Triphenylphosphine-mediated hydroxide formation was used to authenticate the signal generated by the hydroperoxides. The method readily detected lipid peroxidation in a ran...