Defining the mechanical and biochemical determinates of kinesin processivity is important for understanding how diverse kinesins are tuned for specific cellular functions. Because transmission of mechanical forces through the 14–18 amino acid neck linker domain underlies coordinated stepping [1–6], we investigated the role of neck linker length, charge, and structure in kinesin-1 and kinesin-2 ...

Assembly of the cytoskeletal protein FtsZ into a ring-like structure is required for bacterial cell division. Structurally, FtsZ consists of four domains: the globular N-terminal core, a flexible linker, 8-9 conserved residues implicated in interactions with modulatory proteins, and a highly variable set of 4-10 residues at its very C terminus. Largely ignored and distinguished by lack of prima...

in this work, the template, monomer and cross-linker with the ratio of 1:8:40 were used to synthesize molecularly imprinted polymers (mips) for extraction of the bioactive chemical compounds from some traditional herbs as a sorbent material. quercetin, methacrylic acid (maa), trimethylolpropanetrimethacrylate (trim) and tetrahydrofuran (thf) were used as a template, functional monomer, cross-li...

heavy metal toxicity has serious adverse effects on the environment. the metal sequestering characteristics of a novel metal binding peptide (glu-cys) 11 gly+linker+hexahistidine (ec 11 :his 6 ) was investigated to determine if it can absorb cu 2+ or zn 2+ cations. molecular dynamics simulations were carried out using a model of 6 cu 2+ or zn 2+ and other ions enclosed in a fully hydrated simul...

in this article, we describe a photosensitizer (ps) whose ability to generate singlet oxygen (1o2) and fluorescence emission has been designed as tumor responsive. more specifically, the ps consists of a silicon phthalocyanine (sipc) core, axially substituted with two subphthalocyanine (subpc) units, covalently linked by a disulfide linker, which is cleavable in the presence of a strong reducin...

development of yeast artificial chromosome (y ac) vectors, molecular cloning of large segments of chromosomal dna, and their propagation in yeast cells has become feasible. overlapping y ac provides a route to the development of physical maps of entire mammalian chromosomes. a rapid method was developed to isolate and sequence termini of y ac inserts quickly. the y ac clone is digested with a r...

The objective of this study was to develop a novel bacterially-triggered micro-particular system of de-esterified tragacanth (DET) in combination with Eudragit S-100 coated capsules for colon drug delivery of 5-fluorouracil (5-FU) using microemulsion method. The loading study was conducted at different drug-to-polymer ratios and cross-linker concentrations. The maximum loading efficiency was ac...

The objective of this study was to develop a novel bacterially-triggered micro-particular system of de-esterified tragacanth (DET) in combination with Eudragit S-100 coated capsules for colon drug delivery of 5-fluorouracil (5-FU) using microemulsion method. The loading study was conducted at different drug-to-polymer ratios and cross-linker concentrations. The maximum loading efficiency was ac...

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Two of the eight constructs (the triple-knockout 3K0 and S1) were well fit by the diffusion model yielding estimates for D. These are listed as Deff in Table I since the estimated D values are too slow to be explained by free diffusion, and so instead reflect the process of effective diffusion whereby fast binding interactions combine with free diffusion to mimic a slowed diffusion (Sprague et ...