results: of the 50 samples tested by the elisa method, 26 samples (52%) were positive for igg . no samples were positive for igm. of the 50 samples tested by the chemiluminescence method, 28 samples (56%) were positive for igg. no samples were positive for igm. conclusions: a significant relationship between the age of the youngest child and the infection rate was seen. no significant correlati...

background: aflatoxin m1 (afm1) as a carcinogenic and toxic compound has considerable significance from public health viewpoint. the aim of this study was to determine the level of afm1 in pasteurized and uht milk in west-azerbaijan province of iran. methods: a total of 360 commercial milk samples including 220 pasteurized milk and 140 ultra-high-temperature (uht) milk were purchased randomly f...

cholera toxin b subunit (ctb) has been extensively studied as an immunogen, adjuvant, and inducer of oral tolerance in many investigations. production of ctb has been carried out in the bacterial, plant, insect and yeast expression systems. in this study the expression of the ctb containing a 6xhis-tagged was performed by escherichia coli (e.coli) m15. the yield of purified pentameric recombina...

results the optimal conditions were considered to perform elisa. comparison between results obtained from optimized elisa kit and commercial elisa kit showed a good agreement. background haemophilus influenzae type b (hib) infection has high morbidity and mortality rate, especially in children under 5 years of age. enzyme-linked immunosorbent assay (elisa) technique is the most used method to d...

Type2 Diabetes mellitus is a chronic and most common form of diabetes characterized by hyperglycemia arising from problems with the utilization or production insulin. Various agents related to an increased risk developing T2DM include pro-inflammatory cytokines that are critically associated progressive resistance insulin pathogenesis newly diagnosed T2DM. The study aimed possible association T...

background: pan-igg specific monoclonal antibodies (mabs) are essential tools for assessment of humoral immunity, immune deficiency and gammopathy. in this study, four hybridoma clones producing mabs with different specificities for human igg isotypes were established. methods: splenocytes from balb/c mice immunized with fc fractions of human igg were fused with sp2/0 myeloma cells. hybridoma c...

universal vaccination of neonates and children against diphtheria, tetanus and pertussis has had a tremendous impact on the control of these infectious diseases worldwide. immunization by the triple diphtheria, tetanus and whole cell pertussis vaccine (dtwp) has been applied in iran for almost 50 years. periodic assessment of immunogenicity of this vaccine is an important aspect of successful m...

cystic echinococcosis is an important zoonosis in the sheep rising areas of iran. to develop a simple and reliable diagnostic method for echinococcus infection in definitive hosts, e. granulosus polyoclonal antibodies (polyabs) were prepared from adult worm in rabbit. a selected poab was used for coproantigen detection in faecal samples obtained from animals naturally infected with echinococcus...