2003 I Acknowledgements I am very grateful to my supervisor Dr. Rudy Setiono for his insightful suggestions in both the content and presentation of this thesis. It was his encouragement, support and patience that saw me through and I am ever grateful to him. I am full of gratitude to my boss, Dr. Peng Jinrong, for his understanding and support on allowing me to take the part-time Master of Scie...

Foreword The last nine months of my study were dedicated to writing this master thesis. After having determined that I wanted it to be based on the course of Ergodic Theory, that I took in the first semester of the academic year 2003/2004, Karma Dajani suggested the subject of repetitions in number expansions. The subject interested me right from the start. Since in general writing my master th...

i Acknowledgements I would like to thank to Prof.Dr.Gunter Saake for his support and confidence in me and allowing me to work on master thesis in his group. Thank you! I would like thank to my senior supervisor, Dipl.-Inform. Marko Rosenmueller and Dipl.-Inform. Norbert Siegmund, for their encouragement, guidance, fruitful discussions and many other helps. Without their full support, this thesi...

Mussel adhesive proteins have been suggested as a basis for environmentally friendly adhesives for use in aqueous conditions and in medicine. However, attempts to produce functional and economical recombinant mussel adhesive proteins (mainly foot protein type 1) in several systems have failed. Here, the cDNA coding for Mytilus galloprovincialis foot protein type 5 (Mgfp-5) was isolated for the ...

Natural proteins are often confined within their local microenvironments, such as three-dimensional confinement in organelles or two-dimensional confinement in lipid rafts on cytoplasmic membrane. Spatial confinement restricts proteins' entropic freedom, forces their lateral interaction, and induces new properties that the same proteins lack at the soluble state. So far, the phenomenon of envir...

The αvβ6 integrin is up-regulated in cancer and wound healing but it is not generally expressed in healthy adult tissue. There is increasing evidence that it has a role in cancer progression and will be a useful target for antibody-directed cancer therapies. We report a novel recombinant diabody antibody fragment that targets specifically αvβ6 and blocks its function. The diabody was engineered...

We previously expressed VbhTA constructs for lysate AMPylation assays from the pRSFDuet-1 backbone, a plasmid with two multiple cloning sites that each enable the expression of constructs from a PT7 promoter. Our constructs encoded either the VbhT toxin alone (with an N-terminal hexahistidine (His6-)tag) to be expressed from the first multiple cloning site or had additionally the VbhA antitoxin...

In the presence of a low pH environment, the channel-forming T domain of diphtheria toxin undergoes a conformational change that allows for both its own insertion into planar lipid bilayers and the translocation of the toxin's catalytic domain across them. Given that the T domain contributes only three transmembrane segments, and the channel is permeable to ions as large as glucosamine(+) and N...

Mussel adhesive proteins, including the 20-plus variants of foot protein type 3 (fp-3), have been suggested as potential environmentally friendly adhesives for use in aqueous conditions and in medicine. Here we report the novel production of a recombinant Mytilus galloprovincialis foot protein type 3 variant A (Mgfp-3A) fused with a hexahistidine affinity ligand in Escherichia coli and its appr...