In H. polymorpha, there is still a lack of a highly efficient gene disruption method. To help address this issue, we presented a simple and efficient method for both single and multiple gene disruptions in H. polymorpha. The knockout system combined a variation of sticky-end polymerase chain reaction method (SEP), split marker deletion method, co-transformation of single-stranded DNA and mutant...

Saccharomyces cerevisiae (budding yeast) is a powerful eukaryotic model organism ideally suited to high-throughput genetic analyses, which time and again has yielded insights that further our understanding of cell biology processes conserved in humans. Lithium Acetate (LiAc) transformation of yeast with DNA for the purposes of exogenous protein expression (e.g., plasmids) or genome mutation (e....

Diierent interconnection topologies have diierent desirable properties and diierent algorithms run best on diier-ent networks. If the network topology is reconngurable, it can be tailored to suit the particular algorithm being executed. Reconngurability of processor arrays is also important from fault-tolerance point of view. An array processor that is programmable by the user any number of tim...

Diierent interconnection topologies have diierent desirable properties and diierent algorithms run best on diier-ent networks. If the network topology is reconngurable, it can be tailored to suit the particular algorithm being executed. Reconngurability of processor arrays is also important from fault-tolerance point of view. An array processor that is programmable by the user any number of tim...

Service composition enables flexible creation of new services by assembling independent service components. We are focused on the scenario where such composition takes place across the wide-area Internet. We envision independent providers deploying and managing service instances and portal providers composing them to quickly enable new applications in nextgeneration networks. One of the importa...

We report a case of leukemic arthritis (LA) of monocytic differentiation, which presented with spondyloarthritis-like symptoms and a positive human leukocyte antigen-B27, and discuss its potential mechanisms.The patient was admitted because of pain in her right knee and lower back for 18 months. Magnetic resonance imaging showed diffuse hyperintense signal in the bilateral liac bones and bone m...

Introduction: The received dose to the surface of tumor bed is one of the main issues in beast intraopeative radiotherapy (IORT). Totally, the administered dose to the surface is usually lower than that of radiotherapy reference point (distal end of tumor bed). Employing a buildup bolus can effectively increase the surface dose and therefore, eliminate such created cold points ...