AIMS   To perform an international trial to derive alert and action levels for the use of quantitative PCR (qPCR) in the monitoring of Legionella to determine the effectiveness of control measures against legionellae. METHODS AND RESULTS   Laboratories (7) participated from six countries. Legionellae were determined by culture and qPCR methods with comparable detection limits. Systems were mo...

The prevention and control of legionellosis in water supply systems involves environmental comprehensive sampling. Our aim was to investigate occurrence of Legionella spp. in water supply systems in different administrative districts of Riga, and to find out if there exists a correlation between Legionella spp. positive cases and water source, type of sample, sampling point and temperature of h...

outbreaks of legionellosis may be a side effect of institution-water treatment. however, the long-term outcomes and the predictive factors of legionella prevalence in such systems have still not been fully studied. this study was therefore conducted to investigate the prevalence of legionella spp. and to evaluate the role of bacteriological water quality parameters on its prevalence and removal...

Legionella longbeachae serogroup 1 and other Legionella spp. were isolated from 73% of 45 potting soils made in Australia by 13 manufacturers but were not detected in 19 potting soils made in Greece, Switzerland, and the United Kingdom examined between March 1989 and May 1990. Several Legionella species were isolated from a small number of samples of uncomposted pine sawdusts, but it is not kno...

We investigated recreational vehicle (RV) water reservoirs in response to a case of pneumonia in which Legionella pneumophila was cultured both from the patient and a RV reservoir in which he travelled. Water samples processed and cultured at the CDC according to standard protocol were positive for Legionella spp. in 4/17 (24%) faucets, 1/11 (9%) water tanks from 4/20 (20%) RVs from three diffe...

background and objectives: the microbiological monitoring of the water used for haemodialysis is important especially for legionella and non-fermentative bacteria since patients with end stage renal disease (esrd) are suffering from deteriorated function of immune system. materials and methods: a total 50 water and dialysate samples were weekly collected over a period of 10 weeks from 5 sites. ...

The aim of the study was to assess microbial quality of water in dental unit waterlines in a dental school at Mangalore. Methodology: Water samples were taken from tap water for control, air/water syringe and the high speed hand piece of 20 dental units. The samples were transferred to the laboratory and streaked on the culture media namely Mac Conkey agar, Sabourauds dextrose media, Buffered c...

Legionella spp. and Mycobacterium avium complex (MAC) are opportunistic pathogens of public health concern. Hot water systems, including showers, have been identified as a potential source of infection. This paper describes the colonization of Legionella and MAC on the flexible tubing within a model potable shower system, utilizing thermostatic mixing and a flexible shower head. A MAC qPCR meth...

Legionella spp. present in some human-made water systems can cause Legionnaires' disease in susceptible individuals. Although legionellae have been isolated from the natural environment, variations in the organism's abundance over time and its relationship to aquatic microbiota are poorly understood. Here, we investigated the presence and diversity of legionellae through 16S rRNA gene amplicon ...

OBJECTIVES The activities of pazufloxacin and tosufloxacin against Legionella spp. were evaluated in vitro and compared with those of other quinolones, macrolides and azithromycin. METHODS The conventional MICs were determined by the microbroth dilution method. Intracellular activities of drugs were evaluated by a cfu count. The minimal extracellular concentration inhibiting intracellular gro...