In this study, we fabricated PCL-nanoparticles by encapsulating dual drugs as amphotericin B and doxorubicin via double-emulsion solvent evaporation method also incorporated with ligand-lectin for targeting the infested macrophage cells and prove importance against VL. Different independent processing parameters were assessed systematically to enhance the incorporation of the dual agents with d...

BACKGROUND Lectins are carbohydrate binding proteins or glycoproteins that bind reversibly to specific carbohydrates present on the apposing cells, which are responsible for their ability to agglutinate red blood cells, lymphocytes, fibroblasts, etc. Interest in lectins has been intensified due to their carbohydrate specificity as they can be valuable reagents for the investigation of cell surf...

Within the current study, a delivery system based on a novel polymer-lectin conjugate (carbopol-lectin) was evaluated for the oral delivery of therapeutic peptides and proteins. It was demonstrated that covalent attachment of lectin to carbopol does neither decrease nor abolish the specific binding properties of lectin. Bioadhesion studies revealed that liposomes coated with carbopol lectin are...

A soluble lactose-binding lectin with subunit Mr of 14,500 is believed to function by interacting with extracellular glycoconjugates, because it has been detected extracellularly by immunohistochemistry. This localization has been questioned, however, since the lectin lacks a secretion signal sequence, which challenges the contention that it is secreted. We have demonstrated externalization of ...

mannan-binding lectin (mbl) is a vital protein of innate immune system and has two critical functions: complement activation through the lectin pathway and opsonization. mbl deficiency has been classified as the most common inherited immunodeficiency known in humans (about 30% of the population), and is associated with predisposition to infections and high risk of some autoimmune diseases. the ...

b a ckground: the role of lectins is important in interaction between pathogens and mosquito vectors. this study was performed to identify agglutinin activities of protein molecules on the midgut of culex pipiens . m e t hods: c u lex pipiens was reared in insectray condition and the midguts of males and females (blood fed and un- fed) were dissected separately in tris-hcl buffer. the extracts ...

lectin binding histochemistry was performed on the developing sympathetic ganglionic cells to investigate the distribution and density of defined carbohydrate terminals on the cell surface glycoproteins during autonomic system morphogenesis. sprague-dauley rat embryos from 9th gestational day to birth were fixed and paraffinized. serial sections of these specimens were incubated with different ...

abstract background: cholesterol cleft granulomas with clusters of giant cells were noted to be a common feature of non-specific interstitial pneumonia (nsip). objective: this study aimed to define the cell populations involved in the granulomas. methods: the granulomas of 16 patients with cryptogenic fibrosing alveolitis (five cases with the histological features ofnsip, five with those ofuip ...

a castanea crenata agglutinin (abbreviated as cca) was extracted and purified from bitter apple, citrullus colocynthis l., to determine its effects on the demographic parameters of apomyelois ceratoniae zeller. two groups of first larval instars were reared on an artificial diet containing 2% (w/w) of cca and control diets until emergence of adults. two sex ms-chart life table software was used...

The presence of hydrophobic sites in the lectin-I molecule was indicated by hydrophobic probes like 1-anilinonapthalene-8-sulfonic acid (ANS), 2-p-toluidinyl napthalene-6-sulfonic acid (TNS). N-phenyl-1-napthylamine (NA) and rose bengal (RB). This was further confirmed by amino acid modifications in the hydrophobic region of the lectin-I molecule. The binding of ANS, TNS, NA and RB to lectin-I ...