A sensitive latex immunoassay for human serum lipoprotein Lp(a+) is based on direct agglutination by Lp(a+) of latex particles coated with specific antibody. The agglutination is quantified by turbidimetry using a photometer at 360 nm. The stabilization of antibody-coated latex particles by bovine serum albumin occurs under well-defined conditions (pH, concentration of bovine serum albumin, and...

Sera submitted to the Texas Department of Health for the serodiagnosis of Rickettsia typhi were tested by the microimmunofluorescent antibody technique and a new latex agglutination procedure. Results indicated that the latex agglutination test was sensitive and specific and would serve well as a first-line screening test for murine typhus.

A new method for attaching antigens to latex by an avidin-biotin technique is described. The procedure permits control of the amount of antigen attached to the latex and eliminates the need for highly purified antigens and destructive bridging chemicals. The avidin-biotin latex agglutination assay is a simple, rapid test well suited to detection of viral antibody. The sensitivity and specificit...

rheumatoid factors (rf) are autoantibodies with specificity for the fc portion of human igg. production of rf is a characteristic feature of rheumatoid arthritis (ra) and is detectable in high titer in about 90% of these patients. in this study, we measured total igm, iga, igmrf and igarf in serum and synovial fluid of 45 ra patients by elisa and the results were compared with those obtained by...

A comparison is made of tests for thyroglobulin antibody, using gel diffusion, electroprecipitin, bentonite flocculation, tanned red cell agglutination, and latex slide agglutination techniques on sera from cases of Hashimoto's disease and other thyroid disorders. Any increase in gamma globulin was also noted from the serum electrophoretic pattern. The gel diffusion and electroprecipitin tests ...

background: rotavirus is a major cause of morbidity and mortality among children with gastroenteritis. since the discovery of rotaviruses, several techniques have been used for their laboratory diagnosis; those included electron microscopy (em) and enzyme immunoassay. these methods, however, are expensive and not readily available everywhere. we have developed a technique which can be used for ...

The group A rotavirus staphylococcal co-agglutination test was evaluated and its sensitivity and specificity compared with an in-house enzyme-linked immunosorbent assay (ELISA) and a commercial latex agglutination test (Rotalex). In addition, the storage stability of the staphylococcal reagents was ascertained. Examination of 136 clarified suspensions of diarrhoeal faeces by the staphylococcal ...

Brucella canis, a facultative intracellular pathogen, is the causative agent of canine brucellosis. The diagnosis of canine brucellosis is based on bacteriological examination and serological methods, including agglutination and gel diffusion tests. In this study, four recombinant antigens, heat shock protein 60, rhizopine-binding protein, Cu-Zn superoxide dismutase, and hypothetical protein (A...

Morton, Harry E. (University of Pennsylvania, Philadelphia). Mycoplasma-latex agglutination reaction. J. Bacteriol. 92:1196-1205. 1966.-The building up of Mycoplasma cell mass through adsorption to carrier particles as a method for enhancing the agglutination reaction to identify Mycoplasma is described. Mycoplasma cells of human, avian, swine, goat, sewage, and tissue-culture origin were adsor...