Ag processing is a critical step in defining the repertoire of epitope-specific immune responses. In the present study, HIV-1 p55Gag Ag was synthesized as a DNA plasmid with either lysosomal-associated membrane protein-1 (LAMP/gag) or human dendritic cell-LAMP (DC-LAMP/gag) and used to immunize mice. Analysis of the cellular trafficking of these two chimeras demonstrated that both molecules col...

The lack of rapid, affordable, and accurate diagnostic tests represents the primary hurdle affecting malaria surveillance in resource- and expertise-limited areas. Loop-mediated isothermal amplification (LAMP) is a sensitive, rapid, and cheap diagnostic method. Five species-specific LAMP assays were developed based on 18S rRNA gene. Sensitivity and specificity of LAMP results were calculated as...

In this paper a compact representation, 3D Layered, Adaptive-resolution and Multi-perspective Panorama (LAMP), is proposed for representing large scale and 3D scenes with occlusion. Two kinds of 3D LAMP representations are constructed, i.e. the relief-like LAMP and the image-based LAMP, both of which concisely represent almost all the information from a long image sequence. The relief-like LAMP...

Background: Salmonella can cause serious human gastroenteritis and is frequently isolated from various food samples. The cell culturing, immunoassay, and polymerase chain reactions (PCR) are the current methods to detect such pathogenic agents. However, these methods are time-consuming and labor-intensive, and thus unavailable for rapid-monitoring of Salmonella</em...

Aim This study was designed to optimize and apply the use of loop-mediated isothermal amplification (LAMP) as an alternative to conventional polymerase chain reaction (PCR) for the detection of herpesvirus of turkeys (HVT) (FC 126 strain) in vaccinated and non-vaccinated poultry in Nigeria. Materials and Methods HVT positive control (vaccine) was used for optimization of LAMP using six primer...

Salmonella is an intracellular bacterial pathogen that replicates within a membrane-bound vacuole in host cells. The major lysosomal membrane proteins 1 and 2 (LAMP-1 and LAMP-2) are recruited to the Salmonella-containing vacuole as well as Salmonella- associated filaments (Sifs) that emerge from the vacuole. LAMP-1 is a dominant membrane marker for the vacuole and Sifs. Its colocalization with...

We developed a loop-mediated isothermal amplification (LAMP) assay for the detection of Y. pestis by targeting the 3a sequence on chromosome. All 11 species of the genus Yersinia were used to evaluate the specificity of LAMP and PCR, demonstrating that the primers had a high level of specificity. The sensitivity of LAMP or PCR was 2.3 or 23CFU for pure culture, whereas 2.3×104 or 2.3×106CFU for...

The limbic system-associated membrane protein (LAMP) is a cell surface glycoprotein expressed by cortical and subcortical regions of the mammalian CNS that comprise or receive direct projections from limbic system structures. The early and restricted expression of LAMP has led to its postulated role in neural development. Purification and biochemical characterization of LAMP was performed in or...

background and aims: considering difficulties in usual laboratory methods in detection of viral infections, improved dna-based diagnostic techniques are more reliable. loop mediated isothermal amplification method (lamp) is a nucleic acid amplification method that amplifies dna using six primers which has been developed to diagnose viruses as a rapid and high efficiency test. in this study, the...

conclusions results of the current study confirmed that molecular methods such as pcr and lamp were much more sensitive, rapid, and specific than culturing to identify non-culturable coccoid forms of h. pylori in water. results ten copies of targeted dna were required for pcr detection whereas only five copies gave a positive reaction by lamp assay, with 100% specificity. of the 10 isolates ino...