OBJECTIVE To compare serum concentrations of specific IgE and mean papule diameters induced in the immediate skin reactivity test with cow's milk (CM) and its fractions with results of the oral challenge test (OCT), and to establish cutoff points capable of predicting clinical reactivity to CM in patients treated at a referral service. METHODS One hundred and twenty-two children (median of 17...

Cross reactivity between mammalian proteins (cow, goat, ewe, buffalo) has been previously described in both in vitro and in vivo studies. The highest homologies are observed between the milk proteins from cows and other Bovidae, being on average 96.1% for buffalo, 91.1% for ewe, and 87.6% for goat. Lower sequence similarities are associated with proteins contained in milks from Suidae (pigs and...

Infants diagnosed with allergy to cow's milk protein (CMP) are fed extensively hydrolysed cow's milk formulas, modified soy formulas or even amino acid-based formulas. Hydrolysed rice protein infant formulas have become available and have been shown to be well tolerated by these infants. A prospective open, randomized clinical study to compare the clinical tolerance of a new hydrolysed rice pro...

Background The allergists´ tool box in cat allergy management is limited. Clinical studies have shown that holo beta-lactoglobulin (holoBLG) can restore micronutritional deficits atopic immune cells and alleviate allergic symptoms a completely allergen-nonspecific manner. With this study, we aimed to provide proof of principle allergy. Methods A novel challenge protocol for standardized ECARF a...

Oral tolerance is a promising approach for allergy prevention in early life, but it strongly depends on allergen exposure and proper immune environment. Small tolerance-inducing peptides and dietary immunomodulatory components may comprise an attractive method for allergy prevention in at-risk infants. This study aimed to investigate whether early oral exposure to β-lactoglobulin-derived peptid...

: Chymotrypsin- or trypsin V- (a mixture of trypsin and chymotrypsin) digested beta-lactoglobulin (BLG) peptides were prepared and were confirmed to have much less immunoglobulin (lg)G and lgE reactivity compared with intact BLG by IgG inhibition enzymelinked immunosorbent assay and IgE dot blotting. The lymphocyte responses to intact BLG and these peptides were examined using peripheral blood ...

Methods We observed 100 “mother and child” pairs, which were divided into two groups. All children had AD, associated to cow milk protein (CMP) allergy. Lactating mothers from both groups were treated by diet therapy with the replacement of the cow milk to the New Zealand goat milk (1 group: 43 “mother and child” pairs) or non-dairy diet (2 group: 57 “mother and child” pairs). The effect of die...