The aim of this study was to investigate the existence of a β-lactamase gene in a group of multi-drug resistant Acinetobacter baumannii. Twenty strains of multi-drug resistant A. baumannii were isolated. Thirty-four β-lactamase genes and the ISaba1-OXA-23 linkage were analyzed in these strains by polymerase chain reaction (PCR) and verified by DNA sequencing. Three kinds of β-lactamase genes (T...

background :  pathogenic bacteria because of beta-lactam antibiotic resistance genes are dangerous to society. this resistance due to esbl genes, plasmids and transposons that by receiving or mutation occurs. the most important factor beta-lactam antibiotic resistance is beta lactamase enzymes. the purpose of this study was to evaluate the frequency of hospital opportunistic pathogenic bacteria...

Clinical Oligella urethralis isolate COH-1, which was uncommonly resistant to penicillins and narrow-spectrum cephalosporins, was recovered from a 55-year-old patient with a urinary tract infection. Shotgun cloning into Escherichia coli and expression experiments gave recombinant clones expressing either an AmpC beta-lactamase-type phenotype of resistance or a carbenicillin-hydrolyzing beta-lac...

Clinical Oligella urethralis isolate COH-1, which was uncommonly resistant to penicillins and narrowspectrum cephalosporins, was recovered from a 55-year-old patient with a urinary tract infection. Shotgun cloning into Escherichia coli and expression experiments gave recombinant clones expressing either an AmpC -lactamase-type phenotype of resistance or a carbenicillin-hydrolyzing -lactamase-ty...

AIM The aim of this study was to characterize beta-lactamase antimicrobial resistance in Klebsiella and Enterobacter species isolated from healthy and diarrheic dogs in Andhra Pradesh. MATERIALS AND METHODS A total of 136 rectal swabs were collected from healthy (92) and diarrheic (44) dogs, bacteriological cultured for Klebsiella and Enterobacter growth and screened for beta-lactamase antimi...

OBJECTIVES Isolates previously identified as Citrobacter diversus are now known as Citrobacter koseri. We measured sequence variation at the beta-lactamase structural gene among a group of clinical isolates originally identified as C. diversus by API 20E profiling. METHODS beta-Lactamase and 16S rRNA genes were amplified by PCR and sequenced by standard methods. beta-Lactamase induction was a...

AIM To determine the genetic basis and types of beta-lactamase encountered among enterobacterial isolates of wild pets from the animal exhibit. MATERIALS AND METHODS A total of 17 beta-lactamase-producing enterobacteria recovered from fecal samples of wild pet animals were analyzed for a selected beta-lactamase gene by polymerase chain reaction. RESULTS Molecular analysis identified one or ...

Purpose The aim of this study was to identify β-lactamase-producing oral anaerobic bacteria and screen them for the presence of cfxA and BlaTEM genes that are responsible for β-lactamase production and resistance to β-lactam antibiotics. Material and Methods Periodontal pocket debris samples were collected from 48 patients with chronic periodontitis and anaerobically cultured on blood agar plat...

background: there is a growing concern on the impact of the presence of extended-spectrum β-lactamase (esbl) producing escherichia coli isolated from animals on public health. objectives: the aim of this study was to investigate the presence of three classes of esbl genes in e. coli isolates from sheep and broilers at a slaughter in urmia region, iran. methods: a total of 111 e. coli isolates w...